Spectrally encoded microspheres for immunofluorescence analysis
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20.02.2021 |
Sankova N.
Shalaev P.
Semeykina V.
Dolgushin S.
Odintsova E.
Parkhomchuk E.
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Journal of Applied Polymer Science |
10.1002/app.49890 |
0 |
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© 2020 Wiley Periodicals LLC A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis.
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Polymorphic modifications of novel cerium germanide Ce<inf>33.3</inf>Ir<inf>22.2</inf>Ge<inf>44.5</inf> (at.%)
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05.11.2019 |
Gribanov A.
Gribanova S.
Kaczorowski D.
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Journal of Alloys and Compounds |
10.1016/j.jallcom.2019.151695 |
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© 2019 Elsevier B.V. The formation of novel ternary cerium germanide with the chemical composition Ce33.3Ir22.2Ge44.5 (at.%) was established. As derived from powder X-ray diffraction data, the compound crystallizes with two polymorphic modifications. The high-temperature modification, established for as-cast alloy, possesses a tetragonal ThSi2-type structure and can be named as Ce(IrxGe1-x)2, x = 0.32. The low-temperature modification forms in the course of heat treatment at 1073 K, crystallizes with an orthorhombic structure of its own type and can be labeled as Ce3Ir2-xGe4+x, x = 0.02. Both structures belong to the AlB2 structural family and are closely related to each other. In both modifications, statistical mixtures of Ir and Ge atoms occupy same crystallographic sites. As found by means of magnetization measurements, the two structural variants of Ce33.3Ir22.2Ge44.5 (at.%) exhibit very similar magnetic properties. Both polymorphs are Curie-Weiss paramagnets with strong crystalline electric field features observed at low temperatures.
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Synthesis, in vivo and in silico anticonvulsant activity studies of new derivatives of 2-(2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl)acetamide
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15.10.2019 |
El Kayal W.
Shtrygol S.
Zalevskyi S.
Shark A.
Tsyvunin V.
Kovalenko S.
Bunyatyan N.
Perekhoda L.
Severina H.
Georgiyants V.
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European Journal of Medicinal Chemistry |
10.1016/j.ejmech.2019.06.085 |
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© 2019 Elsevier Masson SAS In order to expand the arsenal of biologically active substances of anticonvulsive action by the interaction of 2-(2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl)acetic acid with the corresponding amines in the presence of N,N′-carbonyldiimidazole in the dioxane medium, a systematic series of 2-(2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl)-N-R-acetamides was obtained. A novel approach to synthesis of the key intermediate - 2-(2,4-dioxo-1,4-dihydro-quinazolin-3(2H)-yl)acetic acid was developed. The structure and purity of the resulting substances was confirmed by elemental analysis, 1H NMR, 13C NMR spectroscopy and LC/MS. Based on the results of docking studies using SCIGRESS software, selected compounds with the best affinity for anticonvulsant protein biomes (PDB codes: 4COF, 3F8E and 1 EOU) are promising for experimental studies of anticonvulsant activity. A comparative analysis of the results of molecular docking and in vivo results suggests that there is a positive correlation between scoring protein inhibition and experimental data. Pharmacological studies have revealed the leader compound 2-(2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl)-N-[(2,4-dichlorophenyl)methyl]acet-amide, which improved all the experimental convulsive syndrome rates in mice without motor coordination impairment and may be recommended for further research. The lowest values of the scoring function of the ligand-peptide interaction are obtained for the synthesized compound and сarbonic anhydrase II (gene name CA2) (PDB code 1 EOU), so its inhibition is proposed by us as the most probable mechanism of the anticonvulsive effect of the leader compound.
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A Novel Heterocyclic System Based on Natural Epoxyalantolactone
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01.10.2019 |
Klochkov S.
Pukhov S.
Afanasieva S.
Neganova M.
Ananiev I.
Avila-Rodriguez M.
Tarasov V.
Aliev G.
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Frontiers in Chemistry |
10.3389/fchem.2019.00655 |
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© Copyright © 2019 Klochkov, Pukhov, Afanasieva, Neganova, Ananiev, Avila-Rodriguez, Tarasov and Aliev. Natural sesquiterpene lactones which contain an exocyclic methylene group in the β-position of the lactone ring react readily with N-nucleophiles. When studying the reaction of the natural epoxyalantolactone with the primary amines we demonstrate the formation of a new heterocyclic system—the hydrogenated benzo[g]furo[4,3,2-cd]indol-3(1H)-one. Spectral data on the characteristics of the synthesized compounds are presented. The data on the reaction mechanisms and its applicability for the preparation are discussed.
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Osteosynthesis of intra-and periarticular fractures of the distal humerus
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01.09.2019 |
Al Abdullah M.
Solod E.
Zagorodniy N.
Lazarev A.
Abdulkhabirov M.
Dzhanibekov M.
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International Journal of Pharmaceutical Research |
10.31838/ijpr/2019.11.03.063 |
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© 2019, Advanced Scientific Research. All rights reserved. Intra - and periarticular fractures of the distal humerus comprise from 0.5% to 15.3% of all fractures of the humerus and 3.5 - 5% of all fractures in adults. In recent years, the growing injury, including the localization. Treatment of fractures of the elbow joint is complex and not fully resolved problem of modern traumatology and orthopedics. This is due to the peculiarities of the structure and biomechanics of the elbow joint, predisposition to the occurrence of heterotopic ossification and the development of posttraumatic contractures. Incorrect healing of fractures leads to complications, so you need the exact matching of fragments. Treatment of intra - and periarticular fractures of the distal humerus remains a topical problem in traumatology. Common, both domestic and foreign authors, is the tactics of surgical treatment of such damage. This article presents one of methods of osteosynthesis of the lower third of the humerus is subcutaneous-subfascialosteosynthesis.
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Preparation of plasmonic porous Au@AgVO<inf>3</inf> belt-like nanocomposites with enhanced visible light photocatalytic activity
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18.05.2018 |
Fu H.
Yang X.
Zhang Z.
Wang W.
An X.
Dong Y.
Li X.
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Nanotechnology |
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4 |
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© 2018 IOP Publishing Ltd. This study reports a visible light-driven plasmonic photocatalyst of Au deposited AgVO3 nanocomposites prepared by a hydrothermal method, and further in situ modification of Au nanoparticles by a reducing agent of NaHSO3 in an aqueous solution at room temperature. Various characterization techniques, such as SEM, TEM, XRD, EDS, XPS, and Brunauer-Emmett-Teller, were used to reveal the morphology, composition, and related properties. The results show that belt-like AgVO3 nanoparticles with a width of ∼100 nm were successfully synthesized, and Au nanoparticles with controlled sizes (5-20 nm) were well distributed on the surface of the nanobelts. The UV-vis absorption spectra indicate that the decoration of Au nanoparticles can modulate the optical properties of the nanocomposites, namely, red shift occurs with the increase of Au content. The photocatalytic activities were measured by monitoring the degradation of Rhodamine B (RhB) with the presence of photocatalysts under visible light irradiation. The photodegradation results show that AgVO3 nanobelts exhibit good visible light photocatalytic activities with a degradation efficiency of 98% in 50 min and a reaction rate constant of 0.025 min-1 towards 30 ppm RhB. With the modification of Au nanoparticles, photocatalytic activity basically increases with the molar ratio of Au to V. Among the Au@AgVO3 nanocomposites, the 3% (molar ratio) Au decorated AgVO3 nanobelts showed the highest photocatalytic activity, and the k (0.064 min-1) was almost two times higher than that of the pure AgVO3 nanobelts. This can be attributed to several factors including specific surface areas, optical properties, and the energy band structure of the composites under visible light illumination. These findings may be useful for the practical use of visible light-driven photocatalysts with enhanced photocatalytic efficiencies for environmental remediation.
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Oxidative transformation of N-substituted 3-aminopyrazoles to azopyrazoles using electrogenerated bromine as a mediator
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01.03.2018 |
Lyalin B.
Sigacheva V.
Kokorekin V.
Dutova T.
Rodionov G.
Petrosyan V.
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Russian Chemical Bulletin |
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1 |
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© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The one-pot process of anodic transformation of N-alkyl-3-aminopyrazoles into azopyrazoles under conditions of membraneless electrolysis in an aqueous solution of NaBr was studied for the first time. It was found that under these conditions the aminopyrazoles, which do not have a substituent at position 4, transform into the corresponding 4,4´-dibromoazopyrazoles. The corresponding yield was in the interval of 28—80%, depending on the structure of the products. The transformation of 4-substituted aminopyrazoles resulted in the formation of azopyrazoles in the yields lying within 62—86% when this process was implemented under conditions with the anodically generated Br2 acting as a mediator. A convenient method of anodic synthesis of azopyrazoles in an aqueous medium without the use of additives of chemical oxidants was proposed. The process is environmentally sound and is characterized by a high atom efficiency (>85%).
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Study of plasma-chemical NO-containing gas flow for treatment of wounds and inflammatory processes
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28.02.2018 |
Pekshev A.
Shekhter A.
Vagapov A.
Sharapov N.
Vanin A.
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Nitric Oxide - Biology and Chemistry |
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5 |
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© 2017 This work is aimed at exhaustive and detailed study of chemical, physical and physico-chemical characteristics of NO-containing gas flow (NO-CGF) generated by a plasma-chemical generator of Plason device, which has been used in medical practice for more than 15 years for effectively healing wound and inflammatory conditions with exogenous nitric oxide (NO-therapy). Data was obtained on spatial structure of the gas flow, and values of its local parameters in axial and radial directions, such as nitric oxide content, velocity, temperature and mass flow density of nitric oxide, providing altogether the effectiveness of treatment by the exogenous NO-therapy method, were determined experimentally and by computations. It was demonstrated that plasma-chemical synthesis of NO from atmospheric air in a low direct current (DC) arc provides a high mass flow of nitric oxide at the level of 1.6–1.8 mg/s, while in the area of impact of NO-CGF on the biological tissue, on its axis, NO content is 400–600 ppm, flow velocity about 5 m/s, nitric oxide mass flow density 0.25–0.40 mg/(s·cm2), temperature 40-60 °C. Tendencies were determined for designing new devices for further experimental biological and medical research in the field of NO-therapy: lowering the temperature of NO-CGF to ambient temperature will enable variation, in experiments, of the affecting flow parameters in a wide range up to their maximum values: NO content up to 2000 ppm, velocity up to 20 m/s, nitric oxide mass flow density up to 2.5 mg/(s·cm2).
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Radiological methods in diagnosis, evaluation of the lung resection volume, planning of thoracoplasty and efficacy monitoring of the surgical treatment of expanded destructive pulmonary multi-drug resistant (MDR) tuberculosis
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01.01.2018 |
Giller D.
Ratobylsky G.
Nikitin M.
Koroev V.
Frolova O.
Shekhter A.
Ots O.
Grigoriev Y.
Lavrov V.
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Russian Electronic Journal of Radiology |
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1 |
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© 2018 Russian Electronic Journal of Radiology.All right reserved. Purpose: Demonstration of various radiological methods possibilities in diagnosis, evaluation of the lung resection volume, planning of thoracoplasty and efficacy monitoring of the surgical treatment of destructive pulmonary multi-drug resistant (MDR) tuberculosis (TB). Results: Plain chest film revealed a widespread pulmonary tuberculosis inflammation. With CT application, the nature and prevalence of pulmonary parenchymal involvement was clarified, indications and volume of complex surgical intervention were determined, and surgical treatment efficacy was controlled. Digital tomosynthesis application determined the state of the lung tissue after resection and the volume of the planned postponed thoracoplasty. Discussion: Taking into account the incidence of pulmonary tuberculosis in our country, the similarity of its clinical and radiological picture with a number of other pathological processes, as well as the polymorphism of alterations, it is necessary to know the pathomorphological, clinical and radiological manifestations of this disorder. A reliable assessment of the respiratory organs state according to the radiography data has objective limits due to the presence of a cumulative effect and other factors. So, CT is the priority method in diagnosis, evaluation of lung resection volume, planning of thoracoplasty, and monitoring of surgical treatment efficacy. Due to layer-by-layer visualization, the method of digital tomosynthesis makes it possible to obtain additional information, in comparison with radiographic data, in pulmonary parenchyma state assessment at pre- and postoperative stages, which indicates the prospects of its application in solving this tasks. Conclusion: Due to modern radiological methods in the study of respiratory organs TB, the question of the expediency of surgical treatment or refusal in favor of conservative therapy is solved, which is of particular relevance in patients with MDR TB. Accurate determination of lung resection and thoracoplasty volume significantly reduces the risk of postoperative complications, which contributes to improving the quality of life of the patient. A correct control of respiratory TB surgical treatment efficacy with CT application allows estimating the state of the zone of interest, and timely reveal the relapse of a specific process.
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Cerebrospinal fluid, brain electrolytes balance, and the unsuspected intrinsic property of melanin to dissociate the water molecule
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01.01.2018 |
Herrera A.
Ashraf G.
Arias Esparza M.
Tarasov V.
Chubarev V.
Avila-Rodriguez M.
Makhmutovа A.
Ganash M.
Mosa O.
Hafeez A.
Bachurin S.
Aliev G.
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CNS and Neurological Disorders - Drug Targets |
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0 |
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© 2018 Bentham Science Publishers. Background & Objective: Regulation of composition, volume and turnover of fluids surrounding the brain and damp cells is vital. These fluids transport all substances required for cells and remove the unwanted materials. This regulation tends to act as barrier to prevent free exchange of materials between the brain and blood. There are specific mechanisms concerned with fluid secretion of the controlled composition of the brain, and others responsible for reabsorption eventually to blood and the extracellular fluid whatever their composition is. The current view assumes that choroidal plexuses secrete the major part of Cerebrospinal Fluid (CSF), while the Blood-Brain Barrier (BBB) has a much less contribution to fluid production, generating Interstitial Fluid (ISF) that drains to CSF. The skull is a rigid box; thereby the sum of volumes occupied by the parenchyma with its ISF, related connective tissue, the vascula-ture, the meninges and the CSF must be relatively constant according to the Monroe-Kellie dogma. This constitutes a formidable challenge that normal organisms surpass daily. The ISF and CSF provide water and solutes influx and efflux from cells to these targeted fluids in a quite precise way. Microvessels within the parenchyma are sufficiently close to every cell where diffusion areas for solutes are tiny. Despite this, CSF and ISF exhibit very similar compositions, but differ significantly from blood plasma. Many hydro-philic substances are effectively prevented from the entry into the brain via blood, while others like neurotransmitters are extremely hindered from getting out of the brain. Anatomical principle of the barrier and routes of fluid transfer cannot explain the extraordinary accuracy of fluids and substances needed to enter or leave the brain firmly. There is one aspect that has not been deeply analyzed, despite being prevalent in all the above processes, it is considered a part of the CSF and ISF dynamics. This aspect is the energy necessary to propel them properly in time, form, space, quantity and temporality. Conclusion: The recent hypothesis based on glucose and ATP as sources of energy presents numerous contradictions and controversies. The discovery of the unsuspected intrinsic ability of melanin to dissociate and reform water molecules, similar to the role of chlorophyll in plants, was confirmed in the study of ISF and CSF biology.
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