Acute IL-1RA treatment suppresses the peripheral and central inflammatory response to spinal cord injury
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01.12.2021 |
Yates A.G.
Jogia T.
Gillespie E.R.
Couch Y.
Ruitenberg M.J.
Anthony D.C.
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Journal of Neuroinflammation |
10.1186/s12974-020-02050-6 |
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© 2021, The Author(s). Background: The acute phase response (APR) to CNS insults contributes to the overall magnitude and nature of the systemic inflammatory response. Aspects of this response are thought to drive secondary inflammatory pathology at the lesion site, and suppression of the APR can therefore afford some neuroprotection. In this study, we examined the APR in a mouse model of traumatic spinal cord injury (SCI), along with its relationship to neutrophil recruitment during the immediate aftermath of the insult. We specifically investigated the effect of IL-1 receptor antagonist (IL-1RA) administration on the APR and leukocyte recruitment to the injured spinal cord. Methods: Adult female C57BL/6 mice underwent either a 70kD contusive SCI, or sham surgery, and tissue was collected at 2, 6, 12, and 24 hours post-operation. For IL-1RA experiments, SCI mice received two intraperitoneal injections of human IL-1RA (100mg/kg), or saline as control, immediately following, and 5 hours after impact, and animals were sacrificed 6 hours later. Blood, spleen, liver and spinal cord were collected to study markers of central and peripheral inflammation by flow cytometry, immunohistochemistry and qPCR. Results were analysed by two-way ANOVA or student’s t-test, as appropriate. Results: SCI induced a robust APR, hallmarked by elevated hepatic expression of pro-inflammatory marker genes and a significantly increased neutrophil presence in the blood, liver and spleen of these animals, as early as 2 hours after injury. This peripheral response preceded significant neutrophil infiltration of the spinal cord, which peaked 24 hours post-SCI. Although expression of IL-1RA was also induced in the liver following SCI, its response was delayed compared to IL-1β. Exogenous administration of IL-1RA during this putative therapeutic window was able to suppress the hepatic APR, as evidenced by a reduction in CXCL1 and SAA-2 expression as well as a significant decrease in neutrophil infiltration in both the liver and the injured spinal cord itself. Conclusions: Our data indicate that peripheral administration of IL-1RA can attenuate the APR which in turn reduces immune cell infiltration at the spinal cord lesion site. We propose IL-1RA treatment as a viable therapeutic strategy to minimise the harmful effects of SCI-induced inflammation.
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Combined Lycium babarum polysaccharides and C-phycocyanin increase gastric Bifidobacterium relative abundance and protect against gastric ulcer caused by aspirin in rats
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01.12.2021 |
Hsieh S.Y.
Lian Y.Z.
Lin I.H.
Yang Y.C.
Tinkov A.A.
Skalny A.V.
Chao J.C.J.
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Nutrition and Metabolism |
10.1186/s12986-020-00538-9 |
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© 2021, The Author(s). Background: Non-steroidal anti-inflammatory drugs such as aspirin are used for the treatment of cardiovascular disease. Chronic use of low-dose aspirin is associated with the occurrence of gastric ulcer. The aim of this study was to investigate the healing potential of Lycium barbarum polysaccharides (LBP) from Chinese Goji berry and C-phycocyanin (CPC) from Spirulina platensis on gastric ulcer in rats. Methods: Male Sprague–Dawley rats were divided into five groups: normal, aspirin (700 mg/kg bw), LBP (aspirin + 100 mg/kg bw/d LBP), CPC (aspirin + 50 mg/kg bw/d CPC), and MIX (aspirin + 50 mg/kg bw/d LBP + 25 mg/kg bw/d CPC) groups. Gastric ulcer was developed by daily oral feeding of aspirin for 8 weeks. Treatments were given orally a week before ulcer induction for 9 weeks. Results: The MIX group elevated gastric cyclooxygenase-1, prostaglandin E2, and total nitrite and nitrate levels by 139%, 86%, and 66%, respectively, compared with the aspirin group (p < 0.05). Moreover, the MIX group reduced lipid peroxides malondialdehyde levels by 78% (p < 0.05). The treatment of LBP and/or CPC increased gastric Bifidobacterium relative abundance by 2.5–4.0 times compared with the aspirin group (p < 0.05). Conclusions: We conclude that combined LBP and CPC enhance gastroprotective factors, inhibit lipid peroxidation, and increase gastric Bifidobacterium relative abundance. Combined LBP and CPC have protective potential against gastric ulcer caused by aspirin in rats.
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Early combination therapy with etanercept and methotrexate in JIA patients shortens the time to reach an inactive disease state and remission: results of a double-blind placebo-controlled trial
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01.12.2021 |
Alexeeva E.
Horneff G.
Dvoryakovskaya T.
Denisova R.
Nikishina I.
Zholobova E.
Malievskiy V.
Santalova G.
Stadler E.
Balykova L.
Spivakovskiy Y.
Kriulin I.
Alshevskaya A.
Moskalev A.
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Pediatric Rheumatology |
10.1186/s12969-020-00488-9 |
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© 2021, The Author(s). Background: Remission is the primary objective of treating juvenile idiopathic arthritis (JIA). It is still debatable whether early intensive treatment is superior in terms of earlier achievement of remission. The aim of this study was to evaluate the effectiveness of early etanercept+methotrexate (ETA+MTX) combination therapy versus step-up MTX monotherapy with ETA added in refractory disease. Methods: A multi-centre, double-blind, randomized study in active polyarticular JIA patients treated with either ETA+MTX (n = 35) or placebo+MTX (n = 33) for up to 24 weeks, followed by a 24-week open-label phase. The efficacy endpoints included pedACR30 criteria improvement at week 12, inactive disease at week 24, and remission at week 48. Patients who failed to achieve the endpoints at week 12 or at week 24 escaped to open-label ETA+MTX. Safety was assessed at each visit. Results: By intention-to-treat analysis, more patients in the ETA+MTX group reached the pedACR30 response at week 12 (33 (94.3%)) than in the placebo+MTX group (20 (60.6%); p = 0.001). At week 24, comparable percentages of patients reached inactive disease (11 (31.4%) vs 11 (33.3%)). At week 48, 11 (31.4%) and eight (24.2%) patients achieved remission. The median (+/−IQR) times to achieve an inactive disease state in the ETA+MTX and placebo+MTX groups were 24 (14–32) and 32 (24–40) weeks, respectively. Forty-four (74/100 patient-years) adverse events (AEs) were reported, leading to treatment discontinuation in 6 patients. Conclusions: Early combination therapy with ETA+MTX proved to be highly effective compared to the standard step-up regimen. Compared to those treated with the standard regimen, more patients treated with a combination of ETA+MTX reached the pedACR30 response and achieved inactive disease and remission more rapidly.
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Combined Lycium babarum polysaccharides and C-phycocyanin increase gastric Bifidobacterium relative abundance and protect against gastric ulcer caused by aspirin in rats
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01.12.2021 |
Hsieh S.Y.
Lian Y.Z.
Lin I.H.
Yang Y.C.
Tinkov A.A.
Skalny A.V.
Chao J.C.J.
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Nutrition and Metabolism |
10.1186/s12986-020-00538-9 |
0 |
Ссылка
© 2021, The Author(s). Background: Non-steroidal anti-inflammatory drugs such as aspirin are used for the treatment of cardiovascular disease. Chronic use of low-dose aspirin is associated with the occurrence of gastric ulcer. The aim of this study was to investigate the healing potential of Lycium barbarum polysaccharides (LBP) from Chinese Goji berry and C-phycocyanin (CPC) from Spirulina platensis on gastric ulcer in rats. Methods: Male Sprague–Dawley rats were divided into five groups: normal, aspirin (700 mg/kg bw), LBP (aspirin + 100 mg/kg bw/d LBP), CPC (aspirin + 50 mg/kg bw/d CPC), and MIX (aspirin + 50 mg/kg bw/d LBP + 25 mg/kg bw/d CPC) groups. Gastric ulcer was developed by daily oral feeding of aspirin for 8 weeks. Treatments were given orally a week before ulcer induction for 9 weeks. Results: The MIX group elevated gastric cyclooxygenase-1, prostaglandin E2, and total nitrite and nitrate levels by 139%, 86%, and 66%, respectively, compared with the aspirin group (p < 0.05). Moreover, the MIX group reduced lipid peroxides malondialdehyde levels by 78% (p < 0.05). The treatment of LBP and/or CPC increased gastric Bifidobacterium relative abundance by 2.5–4.0 times compared with the aspirin group (p < 0.05). Conclusions: We conclude that combined LBP and CPC enhance gastroprotective factors, inhibit lipid peroxidation, and increase gastric Bifidobacterium relative abundance. Combined LBP and CPC have protective potential against gastric ulcer caused by aspirin in rats.
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Early combination therapy with etanercept and methotrexate in JIA patients shortens the time to reach an inactive disease state and remission: results of a double-blind placebo-controlled trial
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01.12.2021 |
Alexeeva E.
Horneff G.
Dvoryakovskaya T.
Denisova R.
Nikishina I.
Zholobova E.
Malievskiy V.
Santalova G.
Stadler E.
Balykova L.
Spivakovskiy Y.
Kriulin I.
Alshevskaya A.
Moskalev A.
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Pediatric Rheumatology |
10.1186/s12969-020-00488-9 |
0 |
Ссылка
© 2021, The Author(s). Background: Remission is the primary objective of treating juvenile idiopathic arthritis (JIA). It is still debatable whether early intensive treatment is superior in terms of earlier achievement of remission. The aim of this study was to evaluate the effectiveness of early etanercept+methotrexate (ETA+MTX) combination therapy versus step-up MTX monotherapy with ETA added in refractory disease. Methods: A multi-centre, double-blind, randomized study in active polyarticular JIA patients treated with either ETA+MTX (n = 35) or placebo+MTX (n = 33) for up to 24 weeks, followed by a 24-week open-label phase. The efficacy endpoints included pedACR30 criteria improvement at week 12, inactive disease at week 24, and remission at week 48. Patients who failed to achieve the endpoints at week 12 or at week 24 escaped to open-label ETA+MTX. Safety was assessed at each visit. Results: By intention-to-treat analysis, more patients in the ETA+MTX group reached the pedACR30 response at week 12 (33 (94.3%)) than in the placebo+MTX group (20 (60.6%); p = 0.001). At week 24, comparable percentages of patients reached inactive disease (11 (31.4%) vs 11 (33.3%)). At week 48, 11 (31.4%) and eight (24.2%) patients achieved remission. The median (+/−IQR) times to achieve an inactive disease state in the ETA+MTX and placebo+MTX groups were 24 (14–32) and 32 (24–40) weeks, respectively. Forty-four (74/100 patient-years) adverse events (AEs) were reported, leading to treatment discontinuation in 6 patients. Conclusions: Early combination therapy with ETA+MTX proved to be highly effective compared to the standard step-up regimen. Compared to those treated with the standard regimen, more patients treated with a combination of ETA+MTX reached the pedACR30 response and achieved inactive disease and remission more rapidly.
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Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas
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01.12.2021 |
Kireev D.E.
Chulanov V.P.
Shipulin G.A.
Semenov A.V.
Tivanova E.V.
Kolyasnikova N.M.
Zueva E.B.
Pokrovskiy V.V.
Galli C.
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BMC Infectious Diseases |
10.1186/s12879-020-05695-z |
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© 2021, The Author(s). Background: HIV infection is a major health problem in Russia. We aimed to assess HIV prevalence in different population groups and to compare the characteristics of 4th generation immunoassays from Abbott, Bio-Rad, Vector-Best, Diagnostic Systems, and Medical Biological Unit. Methods: The study included 4452 individuals from the general population (GP), 391 subjects at high risk of HIV infection (HR) and 699 with potentially interfering conditions. HIV positivity was confirmed by immunoblot and by HIV RNA, seroconversion and virus diversity panels were also used. HIV avidity was employed to assess recent infections. Results: The prevalence in GP was 0.40%, higher in males (0.62%) and in people aged < 40 years (0.58%). Patients attending dermo-venereal centers and drug users had a high prevalence (34.1 and 58.8%). Recent infections were diagnosed in 20% of GP and in 4.2% of HR. Assay sensitivity was 100% except for one false negative (99,54%, MBU). Specificity was 99.58–99.89% overall, but as low as 93.26% on HR (Vector-Best). Small differences on early seroconversion were recorded. Only the Abbott assay detected all samples on the viral diversity panel. Conclusion: HIV infection rate in the high-risk groups suggests that awareness and screening campaigns should be enhanced. Fourth generation assays are adequate but performance differences must be considered.
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Metabolic syndrome predicts worse perioperative outcomes in patients treated with radical prostatectomy for non-metastatic prostate cancer
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01.06.2021 |
Luzzago S.
Palumbo C.
Rosiello G.
Pecoraro A.
Deuker M.
Stolzenbach F.
Mistretta F.A.
Tian Z.
Musi G.
Montanari E.
Shariat S.F.
Saad F.
Briganti A.
de Cobelli O.
Karakiewicz P.I.
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Surgical Oncology |
10.1016/j.suronc.2020.12.013 |
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© 2021 Elsevier Ltd Objectives: Metabolic syndrome (MetS) and its components (high blood pressure, BMI≥30, altered fasting glucose, low HDL cholesterol and high triglycerides) may undermine early perioperative outcomes after radical prostatectomy (RP). We tested this hypothesis. Materials & methods: Within the National Inpatient Sample database (2008–2015) we identified RP patients. The effect of MetS was tested in four separate univariable analyses, as well as in multivariable regression models predicting: 1) overall complications, 2) length of stay, 3) total hospital charges and 4) non-home based discharge. All models were weighted and adjusted for clustering, as well as all available patient and hospital characteristics. Results: Of 91,618 patients: 1) 50.2% had high blood pressure, 2) 8.0% had BMI≥30, 3) 13.0% had altered fasting glucose, 4) 22.8% had high triglycerides and 5) 0.03% had low HDL cholesterol. Respectively, one vs. two vs. three vs. four MetS components were recorded in 36.2% vs. 19.0% vs. 5.5% vs. 0.8% patients. Of all patients, 6.3% exhibited ≥3 components and qualified for MetS diagnosis. The rates of MetS increased over time (EAPC:+9.8%; p < 0.001). All four tested MetS components (high blood pressure, BMI≥30, altered fasting glucose and high triglycerides) achieved independent predictor status in all four examined endpoints. Moreover, a highly statistically significant dose-response was also confirmed for all four tested endpoints. Conclusion: MetS and its components consistently and strongly predict early adverse outcomes after RP. Moreover, the strength of the effect was directly proportional to the number of MetS components exhibited by each individual patient, even if formal MetS diagnosis of ≥3 components has not been met.
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Identification of synergistic and antagonistic actions of environmental pollutants: Bisphenols A, S and F in the presence of DEP, DBP, BADGE and BADGE·2HCl in three component mixtures
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01.05.2021 |
Jatkowska N.
Kudłak B.
Lewandowska P.
Liu W.
Williams M.J.
Schiöth H.B.
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Science of the Total Environment |
10.1016/j.scitotenv.2020.144286 |
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© 2020 Elsevier B.V. Ecosystems are facing increased pressure due to the emission of many classes of emerging contaminants. However, very little is known about the interactions of these pollutants, such as bisphenols (BPs), plasticizers or pharmaceuticals. By employing bioluminescent bacteria (Microtox assay), we were able to define interactions between selected emerging pollutants (namely BPA, BPS, BPF, BADGE, BADGE·2HCl, DEP, DBP) in ternary mixtures, at environmentally relevant concentration levels (down to as low as 1.89, 1.42, 3.08, and 0.326 μM for, respectively, BPA, BPF, BPS and BADGE·2HCl). We provide the first systematic analysis of bisphenols and phthalates in three component mixtures. Using this system, we performed toxicity modelling with concentration addition (CA) and independent action (IA) approaches, followed by data interpretation using Model Deviation Ratio (MDR) evaluation. Interestingly, we mathematically and experimentally confirmed a novel synergy between BPA, BADGE and BADGE·2HCl. The synergy of BPA, BADGE and BADGE·2HCl is distinct, with both models suggesting these analytes have a similar mode of action (MOA). Moreover, we unexpectedly found a strong antagonistic impact with DEP, in mixtures containing BPA and BADGE analogues, which is confirmed with both mathematical models. Our study also shows that the impact of BPS and BPF in many mixtures is highly concentration dependent, justifying the necessity to perform mixture studies using wide concentration ranges. Overall, this study demonstrates that bioluminescent bacteria are a relevant model for detecting the synergistic and antagonist actions of environmental pollutants in mixtures, and highlights the importance of analyzing combinations of pollutants in higher order mixtures.
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Comparative analysis of the use of domestic bioresorbable collagen membranes at the closure of postoperative defects of the oral mucosa in an experiment in vivo
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15.04.2021 |
Blagushina N.
Diachkova E.
Volkova M.
Pankush S.
Tarasenko S.
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Biointerface Research in Applied Chemistry |
10.33263/BRIAC112.98049812 |
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© 2020 by the authors. The success of using soft tissue transplants is enough for their spreading in the clinic, but the need to cover the surface, where the transplant was taken from, can be forgotten. It can lead to long-term discomfort of the patient in real life, in some cases to complications alike bleeding; We performed the analysis of the use of different new xenogenic resorbable membranes created within our University in compare with the natural healing of oral mucosa defects in the experiment in vivo on 36 rabbits after performing of the surgical wound on the palatine side (5x5 mm). All animals were separated (divided) for 3 groups: #1 group of control and main groups #2 and #3, where we used pericardium and collagen film for covering mucous defects. We assessed the edema, hyperemia in the operation side, the pain according to animal behavior, the histological picture after animals completion of the experiment (on 3rd, 6th and 10th days); The decrease of clinical signs of inflammation in groups of collagen and pericardium films use (p<0.05) was statistically confirmed. Analysis of histologic investigation of biopsy specimens has shown the faster and massive growth of soft tissue in the donor site after application of pericardium and collagen films (p<0.05). An analysis of the experiment results allows recommending their possible use for closing the donor site after taking a free gingival graft or in the zone of postoperative wound defect in the oral mucosa in clinical oral surgery after specific clinical trials.
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Pulsed laser reshaping and fragmentation of upconversion nanoparticles — from hexagonal prisms to 1D nanorods through “Medusa”-like structures
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01.04.2021 |
Sajti L.
Karimov D.N.
Rocheva V.V.
Arkharova N.A.
Khaydukov K.V.
Lebedev O.I.
Voloshin A.E.
Generalova A.N.
Chichkov B.N.
Khaydukov E.V.
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Nano Research |
10.1007/s12274-020-3163-4 |
0 |
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© 2020, Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature. One dimensional (1D) nanostructures attract considerable attention, enabling a broad application owing to their unique properties. However, the precise mechanism of 1D morphology attainment remains a matter of debate. In this study, ultrafast picosecond (ps) laser-induced treatment on upconversion nanoparticles (UCNPs) is offered as a tool for 1D-nanostructures formation. Fragmentation, reshaping through recrystallization process and bioadaptation of initially hydrophobic (β-Na1.5Y1.5F6: Yb3+, Tm3+/β-Na1.5Y1.5F6) core/shell nanoparticles by means of one-step laser treatment in water are demonstrated. “True” 1D nanostructures through “Medusa”-like structures can be obtained, maintaining anti-Stokes luminescence functionalities. A matter of the one-dimensional UCNPs based on direction of energy migration processes is debated. The proposed laser treatment approach is suitable for fast UCNP surface modification and nano-to-nano transformation, that open unique opportunities to expand UCNP applications in industry and biomedicine. [Figure not available: see fulltext.].
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Ultra-high sensitivity and selectivity of Au nanoparticles modified MoO<inf>3</inf> nanobelts towards 1-butylamine
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15.03.2021 |
Fu H.
Wu Z.
Yang X.
He P.
An X.
Xiong S.
Han D.
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Applied Surface Science |
10.1016/j.apsusc.2020.148721 |
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© 2020 This study demonstrates an ultra-sensitive material towards 1-butylamine. The material is composed of 4 wt% Au nanoparticles decorated on MoO3 nanobelts, which are prepared via the hydrothermal method and in-situ reduction. The related characterizations reveal that the nanobelts are highly crystallized layer structures with a width of ~ 200 nm, a thickness of 40 nm and a length of several micrometers. The Au/MoO3 composites exhibit ultra-high sensing response (~300) towards 100 ppm of 1-butylamine at the working temperature of 240 °C. Even without Au decoration, the pristine MoO3 nanobelts offer the response as high as ~ 90 toward the same concentration of 1-butylamine at the temperature of 340 °C, much higher than the existing materials. More importantly, the proposal materials have excellent selectivity towards 1-butylamine, which offers the possibility for practical use. The excellent sensing performance is attributed to the unique sensing mechanism of the layered MoO3 nanobelts via catalytic reaction between 1-butylamine and the lattice oxygen of MoO3. Besides, Au decoration enables to enhance the adsorption of 1-butylamine and facilitate the catalytic sensing process, resulting in further increase in sensing response and selectivity of 1-butylamine. This study may shield light on a promising high-performance gas sensing materials to detect amines in practical application.
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Laser fabrication of composite layers from biopolymers with branched 3D networks of single-walled carbon nanotubes for cardiovascular implants
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15.03.2021 |
Gerasimenko A.Y.
Kurilova U.E.
Savelyev M.S.
Murashko D.T.
Glukhova O.E.
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Composite Structures |
10.1016/j.compstruct.2020.113517 |
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© 2020 Elsevier Ltd A laser technology has been developed for fabricating structures from composite layers based on biopolymers: albumin, collagen, and chitosan with single-walled carbon nanotubes (SWCNT). The structures are intended for cardiovascular devices and tissue-engineered implants. This is evidenced by the results of studies. The composite layers were fabricated due to the phase transition of biopolymers and SWCNT aqueous dispersion under the influence of laser pulses. At the same time branched 3D networks of SWCNT were formed in the biopolymer matrix. The threshold energy fluence of laser pulses was determined (0.032–0.083 J/cm2) at which a bimodal distribution of pores was observed. The calculation of contact resistances between nanotubes at percolation units of 3D networks (20–100 kOhm) was carried out. Composite layers fabricated by laser demonstrated conductivity values that were higher (12.4 S/m) than those for layers by thermostat (4.7 S/m). The maximum hardness of the composite layers with SWCNT (0.01 wt%) by laser was 482 ± 10, 425 ± 10, and 407 ± 15 MPa for albumin, collagen and chitosan, respectively. The hardness of the thermostat layers was less than 100 MPa. The viability of endothelial cells in composite layers was improved. The composite layers ensured a normal level of hemolysis during interaction with erythrocytes.
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CNS genomic profiling in the mouse chronic social stress model implicates a novel category of candidate genes integrating affective pathogenesis
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08.03.2021 |
Demin K.A.
Smagin D.A.
Kovalenko I.L.
Strekalova T.
Galstyan D.S.
Kolesnikova T.O.
De Abreu M.S.
Galyamina A.G.
Bashirzade A.
Kalueff A.V.
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Progress in Neuro-Psychopharmacology and Biological Psychiatry |
10.1016/j.pnpbp.2020.110086 |
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© 2020 Elsevier Inc. Despite high prevalence, medical impact and societal burden, anxiety, depression and other affective disorders remain poorly understood and treated. Clinical complexity and polygenic nature complicate their analyses, often revealing genetic overlap and cross-disorder heritability. However, the interplay or overlaps between disordered phenotypes can also be based on shared molecular pathways and ‘crosstalk’ mechanisms, which themselves may be genetically determined. We have earlier predicted (Kalueff et al., 2014) a new class of ‘interlinking’ brain genes that do not affect the disordered phenotypes per se, but can instead specifically determine their interrelatedness. To test this hypothesis experimentally, here we applied a well-established rodent chronic social defeat stress model, known to progress in C57BL/6J mice from the Anxiety-like stage on Day 10 to Depression-like stage on Day 20. The present study analyzed mouse whole-genome expression in the prefrontal cortex and hippocampus during the Day 10, the Transitional (Day 15) and Day 20 stages in this model. Our main question here was whether a putative the Transitional stage (Day 15) would reveal distinct characteristic genomic responses from Days 10 and 20 of the model, thus reflecting unique molecular events underlining the transformation or switch from anxiety to depression pathogenesis. Overall, while in the Day 10 (Anxiety) group both brain regions showed major genomic alterations in various neurotransmitter signaling pathways, the Day 15 (Transitional) group revealed uniquely downregulated astrocyte-related genes, and the Day 20 (Depression) group demonstrated multiple downregulated genes of cell adhesion, inflammation and ion transport pathways. Together, these results reveal a complex temporal dynamics of mouse affective phenotypes as they develop. Our genomic profiling findings provide first experimental support to the idea that novel brain genes (activated here only during the Transitional stage) may uniquely integrate anxiety and depression pathogenesis and, hence, determine the progression from one pathological state to another. This concept can potentially be extended to other brain conditions as well. This preclinical study also further implicates cilial and astrocytal mechanisms in the pathogenesis of affective disorders.
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Speciation of essential nutrient trace elements in coconut water
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01.03.2021 |
Alchoubassi G.
Kińska K.
Bierla K.
Lobinski R.
Szpunar J.
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Food Chemistry |
10.1016/j.foodchem.2020.127680 |
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© 2020 Elsevier Ltd Coconut water (Cocos Nucifera) is shown to be a source of essential elements present in the form of low-molecular weight stable complexes known for their bio-availability. The total element concentrations were in the range of 0.2–2.7, 0.3–1, 3–14 and 0.5–2 ppm for Fe, Cu, Mn, and Zn, respectively, and varied as a function of the origin of the nut and its maturity. Speciation was investigated by size-exclusion chromatography - inductively coupled plasma mass spectrometry (ICP MS), and hydrophilic interaction liquid chromatography (HILIC) - electrospray-Orbitrap MS. The metal species identified included: iron complexes with citrate and malate: FeIII(Cit)3(Mal), FeIII(Cit)2(Mal)2, FeIII(Mal)2, glutamine: FeIII(Glu)2 and nicotianamine: FeII(NA); copper complexes with phenylanine: CuII(Phe)2 and CuII(Phe)3 and nicotianamine: CuII(NA); zinc complexes with citrate: ZnII(Cit)2 and nicotianamine ZnII(NA) and manganese complex with asparagine MnII(Asp)2. The contributions of the individual species to the total elements concentrations could be estimated by HILIC – ICP MS.
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Successful endovascular treatment of extra-intracranial arteriovenous malformation using a combination of liquid non-adhesive embolic agents
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01.03.2021 |
Karasev S.M.
Sufianov A.A.
Khafizov R.R.
Karaseva I.I.
Shugushev Z.K.
Maximkin D.A.
Khafizov T.N.
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Interdisciplinary Neurosurgery: Advanced Techniques and Case Management |
10.1016/j.inat.2020.101008 |
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© 2020 The Authors Congenital malformations of blood vessels, including arteriovenous malformations (AVMs), are a less common pathology, which remains one of the most diagnostically and therapeutically complex diseases for treatment. Patients with vascular abnormalities often receive an erroneous diagnosis and inadequate treatment. Depending on the abnormality, such improper treatment can lead to potential long-term functional and cosmetic consequences. Existing treatment options include surgical resection, endovascular embolization, and a combination of these methods. To date, there is no pharmacotherapy available that allows you to radically treat this pathology. Despite recent advances, AVMs are rarely cured and may require multi-stage therapy throughout life. The article presents a clinical case of successful endovascular treatment of extra-intracranial arteriovenous malformation of the frontoparietal region in a young girl with a more pronounced extracranial component of AVM on the front and frontoparietal region with cosmetic and functionally significant defect. We performed double-stage embolization of AVMs with a combination of non-adhesive liquid embolic agents with high and low viscosity. We got good angiographic, clinical and cosmetic treatment results.
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Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia
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01.03.2021 |
Dedkov V.G.
Dolgova A.S.
Safonova M.V.
Samoilov A.E.
Belova O.A.
Kholodilov I.S.
Matsvay A.D.
Speranskaya A.S.
Khafizov K.
Karganova G.G.
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Ticks and Tick-borne Diseases |
10.1016/j.ttbdis.2020.101612 |
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© 2020 Elsevier GmbH Wad Medani virus (WMV) belongs to the genus Orbivirus and is a poorly studied arbovirus with unclear medical significance. Presently, a limited number of WMV strains are characterized and available in NCBI GenBank, some isolated many years ago. A new WMV strain was isolated in 2012 from Dermacentor nuttalli ticks collected from sheep in the Tuva Republic, Russia, and sequenced using high-throughput methods. Complete coding sequences were obtained revealing signs of multiple intersegment reassortments. These point to a high variability potential in WMV that may lead to the formation of strains with novel properties. These new data on WMV can promote better understanding of: ecological features of its circulation; relationships within the genus Orbivirus; and the medical significance of the virus.
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Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia
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01.03.2021 |
Dedkov V.G.
Dolgova A.S.
Safonova M.V.
Samoilov A.E.
Belova O.A.
Kholodilov I.S.
Matsvay A.D.
Speranskaya A.S.
Khafizov K.
Karganova G.G.
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Ticks and Tick-borne Diseases |
10.1016/j.ttbdis.2020.101612 |
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Ссылка
© 2020 Elsevier GmbH Wad Medani virus (WMV) belongs to the genus Orbivirus and is a poorly studied arbovirus with unclear medical significance. Presently, a limited number of WMV strains are characterized and available in NCBI GenBank, some isolated many years ago. A new WMV strain was isolated in 2012 from Dermacentor nuttalli ticks collected from sheep in the Tuva Republic, Russia, and sequenced using high-throughput methods. Complete coding sequences were obtained revealing signs of multiple intersegment reassortments. These point to a high variability potential in WMV that may lead to the formation of strains with novel properties. These new data on WMV can promote better understanding of: ecological features of its circulation; relationships within the genus Orbivirus; and the medical significance of the virus.
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The potential role of emicizumab prophylaxis in severe von Willebrand disease
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01.03.2021 |
Barg A.A.
Avishai E.
Budnik I.
Brutman T.B.
Tamarin I.
Dardik R.
Bashari D.
Misgav M.
Lubetsky A.
Lalezari S.
Livnat T.
Kenet G.
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Blood Cells, Molecules, and Diseases |
10.1016/j.bcmd.2020.102530 |
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© 2020 Elsevier Inc. Background: Severe von Willebrand disease (VWD) may be associated with chronic joint damage and may require prophylactic therapy. Emicizumab is a humanized bispecific antibody, which mimics the function of coagulation factor VIII (FVIII), and it has been approved for prophylaxis in hemophilia A. Methods: This is the first study assessing the potential future role of emicizumab as an alternative prophylactic treatment in patients with severe VWD, based upon a thrombin generation (TG) ex vivo analysis. We report 51 weeks of successful off label emicizumab prophylaxis in a child with severe VWD and recurrent hemarthroses and progressive arthropathy despite adherence to previous prophylaxis with replacement therapy. Results and conclusions: Our work demonstrated that ex vivo spiking with emicizumab increased TG in plasma from patients with type 3 VWD. Similar TG results were observed in our treated patient, whose therapy was well tolerated without any adverse events. Both in vitro and ex vivo TG data support sufficient hemostasis without exceeding the range seen in healthy volunteers. Further collaborative studies on the efficacy and safety of emicizumab prophylaxis in severe VWD is warranted.
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тезис
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The potential role of emicizumab prophylaxis in severe von Willebrand disease
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01.03.2021 |
Barg A.A.
Avishai E.
Budnik I.
Brutman T.B.
Tamarin I.
Dardik R.
Bashari D.
Misgav M.
Lubetsky A.
Lalezari S.
Livnat T.
Kenet G.
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Blood Cells, Molecules, and Diseases |
10.1016/j.bcmd.2020.102530 |
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Ссылка
© 2020 Elsevier Inc. Background: Severe von Willebrand disease (VWD) may be associated with chronic joint damage and may require prophylactic therapy. Emicizumab is a humanized bispecific antibody, which mimics the function of coagulation factor VIII (FVIII), and it has been approved for prophylaxis in hemophilia A. Methods: This is the first study assessing the potential future role of emicizumab as an alternative prophylactic treatment in patients with severe VWD, based upon a thrombin generation (TG) ex vivo analysis. We report 51 weeks of successful off label emicizumab prophylaxis in a child with severe VWD and recurrent hemarthroses and progressive arthropathy despite adherence to previous prophylaxis with replacement therapy. Results and conclusions: Our work demonstrated that ex vivo spiking with emicizumab increased TG in plasma from patients with type 3 VWD. Similar TG results were observed in our treated patient, whose therapy was well tolerated without any adverse events. Both in vitro and ex vivo TG data support sufficient hemostasis without exceeding the range seen in healthy volunteers. Further collaborative studies on the efficacy and safety of emicizumab prophylaxis in severe VWD is warranted.
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Contemporary rates and predictors of open conversion during minimally invasive partial nephrectomy for kidney cancer
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01.03.2021 |
Luzzago S.
Rosiello G.
Pecoraro A.
Deuker M.
Stolzenbach F.
Mistretta F.A.
Tian Z.
Musi G.
Montanari E.
Shariat S.F.
Saad F.
Briganti A.
de Cobelli O.
Karakiewicz P.I.
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Surgical Oncology |
10.1016/j.suronc.2020.12.004 |
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© 2020 Elsevier Ltd Objectives: To test contemporary rates and predictors of open conversion at minimally invasive partial nephrectomy (MIPN: laparoscopic or robotic partial nephrectomy). Materials and methods: Within the National Inpatient Sample database (2008–2015) we identified all MIPN patients and patients that underwent open conversion at MIPN. First, estimated annual percentage changes (EAPC) tested temporal trends of open conversion. Second, univariable and multivariable logistic regression models predicted open conversion at MIPN. All models were weighted and adjusted for clustering, as well as all available patient and hospital characteristics. Results: Of 7649 MIPN patients, 287 (3.8%) underwent open conversion. The rates of open conversion decreased over time (from 12 to 2.4%; EAPC: 24.8%; p = 0.004). In multivariable logistic regression models predicting open conversion, patient obesity achieved independent predictor status (OR:1.80; p < 0.001). Moreover, compared to high volume hospitals, medium volume (OR:1.48; p = 0.02) and low volume hospitals (OR:2.11; p < 0.001) were associated with higher rates of open conversion. Last but not least, when the effect of obesity was tested according to hospital volume, the rates of open conversion ranged from 2.2 (non obese patients treated at high volume hospitals) to 9.8% (obese patients treated at low volume hospitals). Conclusion: Overall contemporary (2008–2015) rate of open conversion at MIPN was 3.8% and it was strongly associated with patient obesity and hospital surgical volume. In consequence, these two parameters should be taken into account during preoperative patients counselling, as well as in clinical and administrative decision making.
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