Analysis of the effect of Nd:YAG laser irradiation on soft tissues of the oral cavity in different modes in an in vivo experiment
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01.01.2022 |
Garipov R.
Elena M.
Diachkova E.
Davtyan A.
Me-Likhova D.
Aygul K.E.
Tarasenko S.
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Biointerface Research in Applied Chemistry |
10.33263/BRIAC123.28812888 |
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The development of laser medicine has led to its use in dentistry further to improve existing treatment methods, including surgical techniques. The variety of lasers allows them to be used for procedures on the soft and bone tissues of the oral cavity as well as on the tissues of the teeth. The short duration of laser pulse action on tissues, selective action on pathological tissues in a sterile surgical field, and activation of local and humoral immunity of the oral cavity provides an increase in the regeneration potential of tissues of the postoperative area, which contributes to the shortening of wound process phases, favorable course of the postoperative period, and shortening of the healing time. Our article presents the experience of using the Nd:YAG laser in different modes in replicating the effect of curettage of periodontal pockets in an experiment on laboratory animals. According to the study results, there was a difference in the healing time of soft tissues after their exposure to several modes of the Nd:YAG laser, which makes it possible to recommend each of them for individual clinical cases.
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Trabecular bone attenuation and velocity assess by ultrasound pulse-echoes
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01.01.2020 |
Rusnak I.
Rosenberg N.
Halevy-Politch J.
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Applied Acoustics |
10.1016/j.apacoust.2019.107007 |
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© 2019 Elsevier Ltd Attenuation and velocity (Speed-of-Sound) in a trabecular bone were calculated and evaluated from ultrasound pulse-echoes detection and their processing, applying a single US transducer that operates in transmit/receive mode. The processing of US pulse-echoes utilized development of algorithms for the attenuation and the Speed-of-Sound in trabecular bone. These ultra-sound pulse-echoes were obtained from the front and rear surfaces of a trabecular bone sample. The motivation for this work was to develop an efficient intraosseous monitoring device that provides the Speed-of-Sound and attenuation intraoperatively and in real-time and therefore can be implemented during a surgery. Ultrasonic measurements were performed in the frequency range of 3.5–6.5 [MHz]. In these experiments, the fresh trabecular bone samples were from sheep femora and humerus. The measuring results were correlated (R2 ≥ 0.95) with those obtained previously, mainly during transmission mode studies. According to the obtained results, the presented method presumably will be utilized as a clinical tool in bone surgical procedures and therefore will be able to provide better outcomes, while monitoring intraoperatively and in real-rime intraosseous trabecular bone attenuation and its velocity: In the next stage of this study, the method will be carried out on human tissues and also intraoperatively, during human clinical trials. It is planned to perform it on long bones/tibia, vertebra, in neurosurgery – for drilling the skull and also in dental implantation surgery.
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The earthworm species Eisenia fetida modulates greenhouse gas release and carbon stabilization after rice straw amendment to a paddy soil
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01.09.2018 |
Zaitsev A.
Gorbunova A.
Korobushkin D.
Degtyarev M.
Zhadova A.
Kostina N.
Gongalsky K.
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European Journal of Soil Biology |
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© 2018 Elsevier Masson SAS We performed a mesocosm experiment with rice paddy soils of three different soil types which were collected in the three major regions of rice production in Russia: Krasnodarsky Krai, the Republic of Kalmykia and Primorsky Krai. We used the earthworm species Eisenia fetida (Savigny 1826), introduced at different densities (0, 2, 4 and 6 individuals per mesocosm with an area of 69.4 cm2 and a soil column height of approximately 10 cm) to assess rice straw decomposition and carbon stabilization in the three soil types. After 20 days of the experiment, straw amendment consistently increased CO2 release from all three soil types. The effect was highest in the soil of Krasnodarsky Krai after a straw amendment was combined with 4 earthworms per mesocosm. The effect of earthworms on CH4 release remained soil type specific but did not demonstrate any synergy with the straw amendment. We also recorded the highest enrichment of soil with carbon from the rice straw when compared with the control mesocosms without straw amendment (the difference was 31.5 mg g−1 soil dwt) under the treatment with four earthworms, and this result was independent of soil type. We conclude that the practice of rice straw reincorporation with the amendment of a certain number of earthworms provides a potentially viable way to recycle crop residue.
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The efficiency of free survey services
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01.01.2018 |
Udovichenko E.
Litvinova T.
Udovichenko A.
Krylova O.
V. Savinova O.
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Journal of Social Sciences Research |
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© 2018 Academic Research Publishing Group. The use of new information technologies in the educational process intensifies the need for analyzing the existing resources suitable for teaching students in conformity with professional standards. The objective of this study is to consider the significance of survey services in teaching students market research, to analyze the efficiency of survey services, to prove their positive impact on the learning process and to determine requirements for educational survey services. The materials of the study were students' reports on conducting consumer surveys using either paper or online questionnaires. The research is based on a pedagogical experiment to analyze the applicability of Internet resources in the educational process. The results of students' research were processed through the comparison of the data obtained. The authors of the article have developed the life cycle of educational survey services. Based on stages of the life cycle, the authors have determined desired functional characteristics of free survey services, which will simplify the task of selecting a suitable educational tool. It has been confirmed that the exclusive use of Internet technologies in teaching students market research cannot be sufficient, and they should be used in combination with basic approaches to a marketing theory. The conducted pedagogical experiment has proved the consistency of Internet technologies as an educational tool. The authors of the article have revealed that despite undeniable advantages of online survey services they also have significant shortcomings, which makes it impossible to completely replace classic methods of collecting market information from this moment on. The introduction of free survey services into the educational process increases teaching efficiency and develops professional competencies within the existing standards. Defining functional requirements of the above-mentioned life cycle, teachers can choose the best educational survey service taking into account the stages students will go through while using it. An extensive use of information technologies is essential to the formation of new teaching materials and the renewal of the existing ones based on the synthesis of the classical theory of marketing and innovations.
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Cytological characteristics of oral cavity tissues of e xperimental animals when using photo polymeric material
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01.01.2018 |
Kolodkina V.
Arutyunov A.
Sirak S.
Ivashchenko V.
Skorikova L.
Andriutsa N.
Tsymbalov O.
Risovanniy S.
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Medical News of North Caucasus |
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© 2018 Stavropol State Medical University. All Rights Reserved. The cytological picture of oral tissues in experimental animals was studied when creating a cariogenic model with a formed artificial defect of hard tissues (Black class V), filled with a restorer filling material Restavrin coated with Easy Glaze sealant and without it. The intensification of the formation of plaque and the manifestation of the inflammatory process in a group of animals with defects, but without coating with sealant was revealed by the 30th day of observation. By this time of the experiment, the number of segmented neutrophils and desquamated epithelium cells sharply increased while the ratio of dead to live cells increased, which increases the degree of cariogenic danger and intensity of inflammation in the area of buccal epithelium, gums and periodontium. A mandatory coating of a photo-composite restoration with a sealant is recommended, which serves as a barrier that protects the filling material from the influence of exogenous factors, preserves the aesthetic and mechanical qualities of composite restorations.
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Differentiated effects of glucosaminylmuramildipeptide on the non-transformed and experimentally transformed phenotype of CD62L <sup>+</sup> CD63 <sup>+</sup> CD66d <sup>+</sup> neutrophilic granulocytes in conventionally healthy people
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01.01.2018 |
Nesterova I.
Malinovskaya V.
Khaydukov S.
Dieu Lien N.
Chudilova G.
Lomtatidze L.
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Medical Immunology (Russia) |
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© 2018, SPb RAACI. Modern studies have shown a high plasticity and phenotypic diversity of neutrophilic granulocytes (NG) provided by different receptors, which are diagnostic markers for the functional capacity of the cell in the course of their activities. We investigated NG from peripheral blood, obtained from healthy people of both sexes aged from 26 to 66 years. Evaluation of the neutrophil membrane receptor expression was carried out by flow cytometry. The relative amount of neutrophilic granulocytes expressing membrane CD62L, CD63, CD66d receptors and the intensity of their expression were determined according to their fluorescence intensities. The surface NG membrane receptors, i.e., CD62L, CD63, CD66d were studied upon the in vitro experimental influence of the following bacterial peptides: N-formyl-methionyl-leucyl-phenylalanine (FMLP, model 1); glucosaminylmuramyldipeptide (GMDP, model 2), and simultaneous incubation of NG blood with fMLP and GMDP (model 3). The in vitro treatment with fMLP in the in vitro model was used to transform the NG phenotype of conventionally healthy subjects, expressing CD62, CD63, CD66d molecules. The treatment caused a significantly decrease in both CD62L and the CD62L expression in relative amounts of neutrophilic granulocytes with a parallel increase of CD63 expression density. The effect of GMDP on the NG phenotype of conditionally healthy subjects did not change the amount of CD62L + NG and CD63 + NG, and did not affect CD62L and CD63 expression density on the surface of NG. However, the amount of CD66d + NG was significantly increased with the unchanged expression of CD66d molecules. GMDP introduced together with the bacterial fMLP peptide was shown to neutralize some features of the NG phenotype transformation caused by fMLP, i.e., the amount of CD62L + NG was restored by 22 % and the CD62L expression density increased significantly. At the same time, GMDP did not correct the negative effect of fMLP upon the number of CD63 + NG and CD66d + NG, and on the CD63 and CD66d expression. Simultaneous addition of fMLP and GMDP did significantly increase the amount of CD66d + NG and expression density of CD63 molecules on the CD63 + NG membrane as compared to intact NG of conditionally healthy subjects. The obtained data are important in order to justify some new immunotherapeutic strategies aimed at correction of the negatively transformed NG phenotype, which accompanies some infectious and inflammatory diseases of bacterial etiology with atypical clinical course.
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