A follow-up study of mucociliary clearance and trace element and mineral status in children with chronic rhinosinusitis before and three months after endoscopic sinus surgery
|
01.12.2021 |
Alekseenko S.I.
Karpischenko S.
Artyushkin S.
Barashkova S.
Karganov M.
Anikin I.
Skalny A.V.
Tinkov A.A.
|
Journal of Trace Elements in Medicine and Biology |
10.1016/j.jtemb.2021.126812 |
0 |
Ссылка
Background: The existing data demonstrate the potential role of trace elements in nasal mucociliary clearance, although the association between trace element and mineral status and ciliary function in children with chronic rhinosinusitis is insufficiently studied. Therefore, the objective of the present study is evaluation of trace element and mineral status and mucociliary function in pediatric CRS patients before and after functional endoscopic sinus surgery. Methods: The present study involved 30 children with chronic rhinosinusitis without nasal polyps. During this follow-up the patients were examined preoperatively (point 0), underwent functional endoscopic sinus surgery, and were repeatedly examined at three months postoperatively (point 1). At both points the patients were subjected to quality-of-life assessment using SNOT-20 questionnaire; endoscopic and computer tomography examination of the nasal sinuses; evaluation of ciliary function and mucosal cytology using high-speed videomicroscopy; assessment of blood count and inflammatory markers; as well as analysis of trace element and mineral levels in whole blood, serum, and hair using inductively-coupled plasma mass-spectrometry. Results: The obtained data demonstrate that endoscopic sinus surgery significantly improved sinonasal pathology in children with chronic rhinosinusitis, as evidenced by significantly reduced Lund-Mackay, Lund-Kennedy, and SNOT-20 scores. At the same time, no significant improvement of ciliary functions or mucosal cytology was observed postoperatively. Trace element status assessment demonstrated that postoperative serum Zn, whole blood Mg and Cu were significantly lower as compared to preoperative values. In contrast, serum Mn and Cr, as well as whole blood Cr and hair Se were characterized by a significant increase at three months postoperatively. Multiple linear regression analysis demonstrated that serum Zn is significantly associated with the number of ciliated cells and cell viability, whereas serum Mn and whole blood Cu concentrations are inversely associated with cell viability and ciliary length, respectively. Hair Se was found to be associated with the number of neutrophils in the mucosa biopsy. Conclusion: Redistribution of trace elements and minerals may at least partially mediate prolonged recovery of mucosal ciliary function in children with chronic rhinosinusitis in three months after functional sinus surgery, although the particular mechanisms of these alterations in trace element levels are to be discovered.
Читать
тезис
|
Reducing the bystander effect via decreasing group size to solve the collective-risk social dilemma
|
01.12.2021 |
Jiang L.L.
Gao J.
Chen Z.
Li W.J.
Kurths J.
|
Applied Mathematics and Computation |
10.1016/j.amc.2021.126445 |
0 |
Ссылка
Collective cooperation is essential to human society, and it exists in many social dilemmas. In the scenario of a collective-risk social dilemma, a group of players have to collectively contribute to a public fund to prevent the tragedy of the commons, such as dangerous climate change, because everybody will lose all their remaining money when the damage happens with a certain probability if the group fails to reach a fixed fundraising target. Yet, it remains largely unclear how the group size affects the probability of reaching the collective target and the mechanism that drives different outcomes of the collective cooperation. Here, we contribute to the literature by exploring the role of group size in the collective-risk social dilemma and the potential underlying mechanism using both model simulations and human experiments. Through simulations we found that the rate of failure for collective cooperation increases for larger groups, along with the arising of bystander effect and a decrease in average contributions, which are confirmed by our experimental observations. We further analyze the patterns of investment behaviors in the experiment setting by categorizing players into cooperators, altruists, and free riders using both a clustering method and a golden standard. We found that altruists who tend to contribute more, rather than cooperators who prefer contributing a fair-share investment, play a crucial role in groups with success outcome in early and/or middle stages of the game. Our results indicate that bystanders are dynamic and their amount depends on the contribution of others. When others contribute less, bystanders also contribute less. If the collective goal is unlikely to achieve, more players choose to be bystanders who strategically contribute less, intriguing the failure of the collective goal. Our findings suggest a potentially effective way to solve the collective-risk social dilemma by reducing the bystander effect through the mechanism design of forming small groups.
Читать
тезис
|
A follow-up study of mucociliary clearance and trace element and mineral status in children with chronic rhinosinusitis before and three months after endoscopic sinus surgery
|
01.12.2021 |
Alekseenko S.I.
Karpischenko S.
Artyushkin S.
Barashkova S.
Karganov M.
Anikin I.
Skalny A.V.
Tinkov A.A.
|
Journal of Trace Elements in Medicine and Biology |
10.1016/j.jtemb.2021.126812 |
0 |
Ссылка
Background: The existing data demonstrate the potential role of trace elements in nasal mucociliary clearance, although the association between trace element and mineral status and ciliary function in children with chronic rhinosinusitis is insufficiently studied. Therefore, the objective of the present study is evaluation of trace element and mineral status and mucociliary function in pediatric CRS patients before and after functional endoscopic sinus surgery. Methods: The present study involved 30 children with chronic rhinosinusitis without nasal polyps. During this follow-up the patients were examined preoperatively (point 0), underwent functional endoscopic sinus surgery, and were repeatedly examined at three months postoperatively (point 1). At both points the patients were subjected to quality-of-life assessment using SNOT-20 questionnaire; endoscopic and computer tomography examination of the nasal sinuses; evaluation of ciliary function and mucosal cytology using high-speed videomicroscopy; assessment of blood count and inflammatory markers; as well as analysis of trace element and mineral levels in whole blood, serum, and hair using inductively-coupled plasma mass-spectrometry. Results: The obtained data demonstrate that endoscopic sinus surgery significantly improved sinonasal pathology in children with chronic rhinosinusitis, as evidenced by significantly reduced Lund-Mackay, Lund-Kennedy, and SNOT-20 scores. At the same time, no significant improvement of ciliary functions or mucosal cytology was observed postoperatively. Trace element status assessment demonstrated that postoperative serum Zn, whole blood Mg and Cu were significantly lower as compared to preoperative values. In contrast, serum Mn and Cr, as well as whole blood Cr and hair Se were characterized by a significant increase at three months postoperatively. Multiple linear regression analysis demonstrated that serum Zn is significantly associated with the number of ciliated cells and cell viability, whereas serum Mn and whole blood Cu concentrations are inversely associated with cell viability and ciliary length, respectively. Hair Se was found to be associated with the number of neutrophils in the mucosa biopsy. Conclusion: Redistribution of trace elements and minerals may at least partially mediate prolonged recovery of mucosal ciliary function in children with chronic rhinosinusitis in three months after functional sinus surgery, although the particular mechanisms of these alterations in trace element levels are to be discovered.
Читать
тезис
|
Reducing the bystander effect via decreasing group size to solve the collective-risk social dilemma
|
01.12.2021 |
Jiang L.L.
Gao J.
Chen Z.
Li W.J.
Kurths J.
|
Applied Mathematics and Computation |
10.1016/j.amc.2021.126445 |
0 |
Ссылка
Collective cooperation is essential to human society, and it exists in many social dilemmas. In the scenario of a collective-risk social dilemma, a group of players have to collectively contribute to a public fund to prevent the tragedy of the commons, such as dangerous climate change, because everybody will lose all their remaining money when the damage happens with a certain probability if the group fails to reach a fixed fundraising target. Yet, it remains largely unclear how the group size affects the probability of reaching the collective target and the mechanism that drives different outcomes of the collective cooperation. Here, we contribute to the literature by exploring the role of group size in the collective-risk social dilemma and the potential underlying mechanism using both model simulations and human experiments. Through simulations we found that the rate of failure for collective cooperation increases for larger groups, along with the arising of bystander effect and a decrease in average contributions, which are confirmed by our experimental observations. We further analyze the patterns of investment behaviors in the experiment setting by categorizing players into cooperators, altruists, and free riders using both a clustering method and a golden standard. We found that altruists who tend to contribute more, rather than cooperators who prefer contributing a fair-share investment, play a crucial role in groups with success outcome in early and/or middle stages of the game. Our results indicate that bystanders are dynamic and their amount depends on the contribution of others. When others contribute less, bystanders also contribute less. If the collective goal is unlikely to achieve, more players choose to be bystanders who strategically contribute less, intriguing the failure of the collective goal. Our findings suggest a potentially effective way to solve the collective-risk social dilemma by reducing the bystander effect through the mechanism design of forming small groups.
Читать
тезис
|
CAR-engineered NK cells; a promising therapeutic option for treatment of hematological malignancies
|
01.12.2021 |
Marofi F.
Saleh M.M.
Rahman H.S.
Suksatan W.
Al-Gazally M.E.
Abdelbasset W.K.
Thangavelu L.
Yumashev A.V.
Hassanzadeh A.
Yazdanifar M.
Motavalli R.
Pathak Y.
Naimi A.
Baradaran B.
Nikoo M.
Khiavi F.M.
|
Stem Cell Research and Therapy |
10.1186/s13287-021-02462-y |
0 |
Ссылка
Adoptive cell therapy has received a great deal of interest in the treatment of advanced cancers that are resistant to traditional therapy. The tremendous success of chimeric antigen receptor (CAR)-engineered T (CAR-T) cells in the treatment of cancer, especially hematological cancers, has exposed CAR’s potential. However, the toxicity and significant limitations of CAR-T cell immunotherapy prompted research into other immune cells as potential candidates for CAR engineering. NK cells are a major component of the innate immune system, especially for tumor immunosurveillance. They have a higher propensity for immunotherapy in hematologic malignancies because they can detect and eliminate cancerous cells more effectively. In comparison to CAR-T cells, CAR-NK cells can be prepared from allogeneic donors and are safer with a lower chance of cytokine release syndrome and graft-versus-host disease, as well as being a more efficient antitumor activity with high efficiency for off-the-shelf production. Moreover, CAR-NK cells may be modified to target various antigens while also increasing their expansion and survival in vivo. Extensive preclinical research has shown that NK cells can be effectively engineered to express CARs with substantial cytotoxic activity against both hematological and solid tumors, establishing evidence for potential clinical trials of CAR-NK cells. In this review, we discuss recent advances in CAR-NK cell engineering in a variety of hematological malignancies, as well as the main challenges that influence the outcomes of CAR-NK cell-based tumor immunotherapies.
Читать
тезис
|
A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine
|
01.12.2021 |
Moghadasi S.
Elveny M.
Rahman H.S.
Suksatan W.
Jalil A.T.
Abdelbasset W.K.
Yumashev A.V.
Shariatzadeh S.
Motavalli R.
Behzad F.
Marofi F.
Hassanzadeh A.
Pathak Y.
Jarahian M.
|
Journal of Translational Medicine |
10.1186/s12967-021-02980-6 |
0 |
Ссылка
Recently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs are not related to their differentiation potentials, but rather are exerted through the release of soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, and owning targeting capability and organotropism, exosomes are considered nanocarriers for their possible use in diagnosis and therapy. Exosomes convey functional molecules such as long non-coding RNAs (lncRNAs) and micro-RNAs (miRNAs), proteins (e.g., chemokine and cytokine), and lipids from MSCs to the target cells. They participate in intercellular interaction procedures and enable the repair of damaged or diseased tissues and organs. Findings have evidenced that exosomes alone are liable for the beneficial influences of MSCs in a myriad of experimental models, suggesting that MSC- exosomes can be utilized to establish a novel cell-free therapeutic strategy for the treatment of varied human disorders, encompassing myocardial infarction (MI), CNS-related disorders, musculoskeletal disorders (e.g. arthritis), kidney diseases, liver diseases, lung diseases, as well as cutaneous wounds. Importantly, compared with MSCs, MSC- exosomes serve more steady entities and reduced safety risks concerning the injection of live cells, such as microvasculature occlusion risk. In the current review, we will discuss the therapeutic potential of MSC- exosomes as an innovative approach in the context of regenerative medicine and highlight the recent knowledge on MSC- exosomes in translational medicine, focusing on in vivo researches.
Читать
тезис
|
The mystery of claustral neural circuits and recent updates on its role in neurodegenerative pathology
|
01.12.2021 |
Nikolenko V.N.
Rizaeva N.A.
Beeraka N.M.
Oganesyan M.V.
Kudryashova V.A.
Dubovets A.A.
Borminskaya I.D.
Bulygin K.V.
Sinelnikov M.Y.
Aliev G.
|
Behavioral and Brain Functions |
10.1186/s12993-021-00181-1 |
0 |
Ссылка
Introduction: The claustrum is a structure involved in formation of several cortical and subcortical neural microcircuits which may be involved in such functions as conscious sensations and rewarding behavior. The claustrum is regarded as a multi-modal information processing network. Pathology of the claustrum is seen in certain neurological disorders. To date, there are not enough comprehensive studies that contain accurate information regarding involvement of the claustrum in development of neurological disorders. Objective: Our review aims to provide an update on claustrum anatomy, ontogenesis, cytoarchitecture, neural networks and their functional relation to the incidence of neurological diseases. Materials and methods: A literature review was conducted using the Google Scholar, PubMed, NCBI MedLine, and eLibrary databases. Results: Despite new methods that have made it possible to study the claustrum at the molecular, genetic and epigenetic levels, its functions and connectivity are still poorly understood. The anatomical location, relatively uniform cytoarchitecture, and vast network of connections suggest a divergent role of the claustrum in integration and processing of input information and formation of coherent perceptions. Several studies have shown changes in the appearance, structure and volume of the claustrum in neurodegenerative diseases, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), autism, schizophrenia, and depressive disorders. Taking into account the structure, ontogenesis, and functions of the claustrum, this literature review offers insight into understanding the crucial role of this structure in brain function and behavior.
Читать
тезис
|
A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine
|
01.12.2021 |
Moghadasi S.
Elveny M.
Rahman H.S.
Suksatan W.
Jalil A.T.
Abdelbasset W.K.
Yumashev A.V.
Shariatzadeh S.
Motavalli R.
Behzad F.
Marofi F.
Hassanzadeh A.
Pathak Y.
Jarahian M.
|
Journal of Translational Medicine |
10.1186/s12967-021-02980-6 |
0 |
Ссылка
Recently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs are not related to their differentiation potentials, but rather are exerted through the release of soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, and owning targeting capability and organotropism, exosomes are considered nanocarriers for their possible use in diagnosis and therapy. Exosomes convey functional molecules such as long non-coding RNAs (lncRNAs) and micro-RNAs (miRNAs), proteins (e.g., chemokine and cytokine), and lipids from MSCs to the target cells. They participate in intercellular interaction procedures and enable the repair of damaged or diseased tissues and organs. Findings have evidenced that exosomes alone are liable for the beneficial influences of MSCs in a myriad of experimental models, suggesting that MSC- exosomes can be utilized to establish a novel cell-free therapeutic strategy for the treatment of varied human disorders, encompassing myocardial infarction (MI), CNS-related disorders, musculoskeletal disorders (e.g. arthritis), kidney diseases, liver diseases, lung diseases, as well as cutaneous wounds. Importantly, compared with MSCs, MSC- exosomes serve more steady entities and reduced safety risks concerning the injection of live cells, such as microvasculature occlusion risk. In the current review, we will discuss the therapeutic potential of MSC- exosomes as an innovative approach in the context of regenerative medicine and highlight the recent knowledge on MSC- exosomes in translational medicine, focusing on in vivo researches.
Читать
тезис
|
The mystery of claustral neural circuits and recent updates on its role in neurodegenerative pathology
|
01.12.2021 |
Nikolenko V.N.
Rizaeva N.A.
Beeraka N.M.
Oganesyan M.V.
Kudryashova V.A.
Dubovets A.A.
Borminskaya I.D.
Bulygin K.V.
Sinelnikov M.Y.
Aliev G.
|
Behavioral and Brain Functions |
10.1186/s12993-021-00181-1 |
0 |
Ссылка
Introduction: The claustrum is a structure involved in formation of several cortical and subcortical neural microcircuits which may be involved in such functions as conscious sensations and rewarding behavior. The claustrum is regarded as a multi-modal information processing network. Pathology of the claustrum is seen in certain neurological disorders. To date, there are not enough comprehensive studies that contain accurate information regarding involvement of the claustrum in development of neurological disorders. Objective: Our review aims to provide an update on claustrum anatomy, ontogenesis, cytoarchitecture, neural networks and their functional relation to the incidence of neurological diseases. Materials and methods: A literature review was conducted using the Google Scholar, PubMed, NCBI MedLine, and eLibrary databases. Results: Despite new methods that have made it possible to study the claustrum at the molecular, genetic and epigenetic levels, its functions and connectivity are still poorly understood. The anatomical location, relatively uniform cytoarchitecture, and vast network of connections suggest a divergent role of the claustrum in integration and processing of input information and formation of coherent perceptions. Several studies have shown changes in the appearance, structure and volume of the claustrum in neurodegenerative diseases, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), autism, schizophrenia, and depressive disorders. Taking into account the structure, ontogenesis, and functions of the claustrum, this literature review offers insight into understanding the crucial role of this structure in brain function and behavior.
Читать
тезис
|
Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats
|
01.12.2021 |
Sretenovic J.
Joksimovic Jovic J.
Srejovic I.
Zivkovic V.
Mihajlovic K.
Labudovic-Borovic M.
Trifunovic S.
Milosevic V.
Lazic D.
Bolevich S.
Jakovljevic V.
Milosavljevic Z.
|
Basic and Clinical Andrology |
10.1186/s12610-021-00134-8 |
0 |
Ссылка
Background: During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). Results: Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. Conclusions: Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction.
Читать
тезис
|
Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats
|
01.12.2021 |
Sretenovic J.
Joksimovic Jovic J.
Srejovic I.
Zivkovic V.
Mihajlovic K.
Labudovic-Borovic M.
Trifunovic S.
Milosevic V.
Lazic D.
Bolevich S.
Jakovljevic V.
Milosavljevic Z.
|
Basic and Clinical Andrology |
10.1186/s12610-021-00134-8 |
0 |
Ссылка
Background: During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). Results: Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. Conclusions: Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction.
Читать
тезис
|
Burden and attitude to resistant and refractory migraine: a survey from the European Headache Federation with the endorsement of the European Migraine & Headache Alliance
|
01.12.2021 |
Sacco S.
Lampl C.
Maassen van den Brink A.
Caponnetto V.
Braschinsky M.
Ducros A.
Little P.
Pozo-Rosich P.
Reuter U.
Ruiz de la Torre E.
Sanchez Del Rio M.
Sinclair A.J.
Martelletti P.
Katsarava Z.
Cakciri G.
Djamandi P.
Grabova S.
Halili G.
Kruja J.
Kuqo A.
Naco D.
Quka A.
Stefanidhi L.
Vyshka G.
Zekja I.
Bruera O.
Gómez D.
Guitian B.
Roma J.C.
Chen I.L.
Bashirova S.
Linkov M.
Van Den Abbeele D.
Vanderschueren G.
Araujo R.
Arruda R.
Catharino A.
Ciriaco J.
Dalla Corte A.
Dornas R.
Felsenfeld B.
Fonseca Taufner A.
Fragoso Y.
Hurtado R.
Isoni Martins D.
Londero R.
Melo L.
Mignoni K.S.
Sgobbi De Souza P.V.
Souza M.N.
Osman S.
Baltzer V.
Pacheco Mosquera L.F.
Dubroja I.
Hucika Z.
Lisak M.
Lovrencic-Huzjan A.
Lušic I.
Mahovic Lakusic D.
Mikulenka P.
Rehulka P.
Amin F.M.
Antic S.
Fakhril-Din Z.
Moeller-Hansen J.
Munksgaard S.
Nan A.M.
Pellesi L.
Schytz H.
Vides M.
Braschinsky K.
Krikmann Ü.
Roos C.
Cauchie A.
Christian L.
Guégan-Massardier E.
Demarquay G.
Gilles G.
Mawet J.
Kuhn E.
Lanteri Minet M.
Bustuchina Vlaicu M.
Moisset X.
Muresan M.
Najjar-Ravan M.
Giraud P.
Simonin S.
De Gaalon S.
Chakhava G.
Demuria M.
Gegelashvili G.
Kapanadze N.
Antonakakis A.
Gaul C.
Förderreuther S.
Huhn J.I.
Ibragimov S.
Kamm K.
Raffaelli B.
Czaniera R.
|
Journal of Headache and Pain |
10.1186/s10194-021-01252-4 |
0 |
Ссылка
Background: New treatments are currently offering new opportunities and challenges in clinical management and research in the migraine field. There is the need of homogenous criteria to identify candidates for treatment escalation as well as of reliable criteria to identify refractoriness to treatment. To overcome those issues, the European Headache Federation (EHF) issued a Consensus document to propose criteria to approach difficult-to-treat migraine patients in a standardized way. The Consensus proposed well-defined criteria for resistant migraine (i.e., patients who do not respond to some treatment but who have residual therapeutic opportunities) and refractory migraine (i.e., patients who still have debilitating migraine despite maximal treatment efforts). The aim of this study was to better understand the perceived impact of resistant and refractory migraine and the attitude of physicians involved in migraine care toward those conditions. Methods: We conducted a web-questionnaire-based cross-sectional international study involving physicians with interest in headache care. Results: There were 277 questionnaires available for analysis. A relevant proportion of participants reported that patients with resistant and refractory migraine were frequently seen in their clinical practice (49.5% for resistant and 28.9% for refractory migraine); percentages were higher when considering only those working in specialized headache centers (75% and 46% respectively). However, many physicians reported low or moderate confidence in managing resistant (8.1% and 43.3%, respectively) and refractory (20.7% and 48.4%, respectively) migraine patients; confidence in treating resistant and refractory migraine patients was different according to the level of care and to the number of patients visited per week. Patients with resistant and refractory migraine were infrequently referred to more specialized centers (12% and 19%, respectively); also in this case, figures were different according to the level of care. Conclusions: This report highlights the clinical relevance of difficult-to-treat migraine and the presence of unmet needs in this field. There is the need of more evidence regarding the management of those patients and clear guidance referring to the organization of care and available opportunities.
Читать
тезис
|
Inhaled iloprost improves gas exchange in patients with COVID-19 and acute respiratory distress syndrome
|
01.12.2021 |
Tsareva N.A.
Avdeev S.N.
Kosanovic D.
Schermuly R.T.
Trushenko N.V.
Nekludova G.V.
|
Critical Care |
10.1186/s13054-021-03690-7 |
0 |
Ссылка
|
Structured Q1 headache services as the solution to the ill-health burden of headache: 1. Rationale and description
|
01.12.2021 |
Steiner T.J.
Jensen R.
Katsarava Z.
Stovner L.J.
Uluduz D.
Adarmouch L.
Al Jumah M.
Al Khathaami A.M.
Ashina M.
Braschinsky M.
Broner S.
Eliasson J.H.
Gil-Gouveia R.
Gómez-Galván J.B.
Gudmundsson L.S.
Herekar A.A.
Kawatu N.
Kissani N.
Kulkarni G.B.
Lebedeva E.R.
Leonardi M.
Linde M.
Luvsannorov O.
Maiga Y.
Milanov I.
Mitsikostas D.D.
Musayev T.
Olesen J.
Osipova V.
Paemeleire K.
Peres M.F.P.
Quispe G.
Rao G.N.
Risal A.
de la Torre E.R.
Saylor D.
Togha M.
Yu S.Y.
Zebenigus M.
Zewde Y.Z.
Zidverc-Trajković J.
Tinelli M.
|
Journal of Headache and Pain |
10.1186/s10194-021-01265-z |
1 |
Ссылка
In countries where headache services exist at all, their focus is usually on specialist (tertiary) care. This is clinically and economically inappropriate: most headache disorders can effectively and more efficiently (and at lower cost) be treated in educationally supported primary care. At the same time, compartmentalizing divisions between primary, secondary and tertiary care in many health-care systems create multiple inefficiencies, confronting patients attempting to navigate these levels (the “patient journey”) with perplexing obstacles. High demand for headache care, estimated here in a needs-assessment exercise, is the biggest of the challenges to reform. It is also the principal reason why reform is necessary. The structured headache services model presented here by experts from all world regions on behalf of the Global Campaign against Headache is the suggested health-care solution to headache. It develops and refines previous proposals, responding to the challenge of high demand by basing headache services in primary care, with two supporting arguments. First, only primary care can deliver headache services equitably to the large numbers of people needing it. Second, with educational supports, they can do so effectively to most of these people. The model calls for vertical integration between care levels (primary, secondary and tertiary), and protection of the more advanced levels for the minority of patients who need them. At the same time, it is amenable to horizontal integration with other care services. It is adaptable according to the broader national or regional health services in which headache services should be embedded. It is, according to evidence and argument presented, an efficient and cost-effective model, but these are claims to be tested in formal economic analyses.
Читать
тезис
|
A deep insight into CRISPR/Cas9 application in CAR-T cell-based tumor immunotherapies
|
01.12.2021 |
Razeghian E.
Nasution M.K.M.
Rahman H.S.
Gardanova Z.R.
Abdelbasset W.K.
Aravindhan S.
Bokov D.O.
Suksatan W.
Nakhaei P.
Shariatzadeh S.
Marofi F.
Yazdanifar M.
Shamlou S.
Motavalli R.
Khiavi F.M.
|
Stem Cell Research and Therapy |
10.1186/s13287-021-02510-7 |
0 |
Ссылка
To date, two chimeric antigen receptors (CAR)-T cell products from autologous T cells have been approved by The United States Food and Drug Administration (FDA). The case-by-case autologous T cell generation setting is largely considered as a pivotal restraining cause for its large-scale clinical use because of the costly and prolonged manufacturing procedure. Further, activated CAR-T cells mainly express immune checkpoint molecules, including CTLA4, PD1, LAG3, abrogating CAR-T anti-tumor activity. In addition, CAR-T cell therapy potently results in some toxicity, such as cytokine releases syndrome (CRS). Therefore, the development of the universal allogeneic T cells with higher anti-tumor effects is of paramount importance. Thus, genome-editing technologies, in particular, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 are currently being used to establish “off-the-shelf” CAR-T cells with robust resistance to immune cell-suppressive molecules. In fact, that simultaneous ablation of PD-1, T cell receptor alpha constant (TRAC or TCR), and also β-2 microglobulin (B2M) by CRISPR-Cas9 technique can support the manufacture of universal CAR-T cells with robust resistance to PD-L1. Indeed, the ablation of β2M or TARC can severely hinder swift elimination of allogeneic T cells those express foreign HLA-I molecules, and thereby enables the generation of CAR-T cells from allogeneic healthy donors T cells with higher persistence in vivo. Herein, we will deliver a brief overview of the CAR-T cell application in the context of tumor immunotherapy. More importantly, we will discuss recent finding concerning the application of genome editing technologies for preparing universal CAR-T cells or cells that can effectively counter tumor escape, with a special focus on CRISPR-Cas9 technology.
Читать
тезис
|
A deep insight into CRISPR/Cas9 application in CAR-T cell-based tumor immunotherapies
|
01.12.2021 |
Razeghian E.
Nasution M.K.M.
Rahman H.S.
Gardanova Z.R.
Abdelbasset W.K.
Aravindhan S.
Bokov D.O.
Suksatan W.
Nakhaei P.
Shariatzadeh S.
Marofi F.
Yazdanifar M.
Shamlou S.
Motavalli R.
Khiavi F.M.
|
Stem Cell Research and Therapy |
10.1186/s13287-021-02510-7 |
0 |
Ссылка
To date, two chimeric antigen receptors (CAR)-T cell products from autologous T cells have been approved by The United States Food and Drug Administration (FDA). The case-by-case autologous T cell generation setting is largely considered as a pivotal restraining cause for its large-scale clinical use because of the costly and prolonged manufacturing procedure. Further, activated CAR-T cells mainly express immune checkpoint molecules, including CTLA4, PD1, LAG3, abrogating CAR-T anti-tumor activity. In addition, CAR-T cell therapy potently results in some toxicity, such as cytokine releases syndrome (CRS). Therefore, the development of the universal allogeneic T cells with higher anti-tumor effects is of paramount importance. Thus, genome-editing technologies, in particular, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 are currently being used to establish “off-the-shelf” CAR-T cells with robust resistance to immune cell-suppressive molecules. In fact, that simultaneous ablation of PD-1, T cell receptor alpha constant (TRAC or TCR), and also β-2 microglobulin (B2M) by CRISPR-Cas9 technique can support the manufacture of universal CAR-T cells with robust resistance to PD-L1. Indeed, the ablation of β2M or TARC can severely hinder swift elimination of allogeneic T cells those express foreign HLA-I molecules, and thereby enables the generation of CAR-T cells from allogeneic healthy donors T cells with higher persistence in vivo. Herein, we will deliver a brief overview of the CAR-T cell application in the context of tumor immunotherapy. More importantly, we will discuss recent finding concerning the application of genome editing technologies for preparing universal CAR-T cells or cells that can effectively counter tumor escape, with a special focus on CRISPR-Cas9 technology.
Читать
тезис
|
A deep insight into CRISPR/Cas9 application in CAR-T cell-based tumor immunotherapies
|
01.12.2021 |
Razeghian E.
Nasution M.K.M.
Rahman H.S.
Gardanova Z.R.
Abdelbasset W.K.
Aravindhan S.
Bokov D.O.
Suksatan W.
Nakhaei P.
Shariatzadeh S.
Marofi F.
Yazdanifar M.
Shamlou S.
Motavalli R.
Khiavi F.M.
|
Stem Cell Research and Therapy |
10.1186/s13287-021-02510-7 |
0 |
Ссылка
To date, two chimeric antigen receptors (CAR)-T cell products from autologous T cells have been approved by The United States Food and Drug Administration (FDA). The case-by-case autologous T cell generation setting is largely considered as a pivotal restraining cause for its large-scale clinical use because of the costly and prolonged manufacturing procedure. Further, activated CAR-T cells mainly express immune checkpoint molecules, including CTLA4, PD1, LAG3, abrogating CAR-T anti-tumor activity. In addition, CAR-T cell therapy potently results in some toxicity, such as cytokine releases syndrome (CRS). Therefore, the development of the universal allogeneic T cells with higher anti-tumor effects is of paramount importance. Thus, genome-editing technologies, in particular, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 are currently being used to establish “off-the-shelf” CAR-T cells with robust resistance to immune cell-suppressive molecules. In fact, that simultaneous ablation of PD-1, T cell receptor alpha constant (TRAC or TCR), and also β-2 microglobulin (B2M) by CRISPR-Cas9 technique can support the manufacture of universal CAR-T cells with robust resistance to PD-L1. Indeed, the ablation of β2M or TARC can severely hinder swift elimination of allogeneic T cells those express foreign HLA-I molecules, and thereby enables the generation of CAR-T cells from allogeneic healthy donors T cells with higher persistence in vivo. Herein, we will deliver a brief overview of the CAR-T cell application in the context of tumor immunotherapy. More importantly, we will discuss recent finding concerning the application of genome editing technologies for preparing universal CAR-T cells or cells that can effectively counter tumor escape, with a special focus on CRISPR-Cas9 technology.
Читать
тезис
|
Risky sexual behaviour among Russian adolescents: association with internalizing and externalizing symptoms
|
01.12.2021 |
Isaksson J.
Westermark C.
Koposov R.A.
Stickley A.
Ruchkin V.
|
Child and Adolescent Psychiatry and Mental Health |
10.1186/s13034-021-00393-3 |
0 |
Ссылка
Background: Risky sexual behaviour (RSB) is regarded as a major health problem during adolescence. Russia has one of the highest rates of teenage pregnancy, abortion and newly diagnosed HIV infections in the world, but research on RSB in Russian youth has been limited. To address this deficit, this study examined the role of several factors, including internalizing and externalizing symptoms, in RSB among Russian adolescents. Methods: Self-reported data were collected from 2573 Russian adolescents aged 13–17 years old (59.4 % girls; Mean age = 14.89) regarding RSB (unprotected sex, early pregnancy, multiple sexual partners and substance use during sexual encounters). Information was also obtained on externalizing (conduct problems and delinquent behaviour) and internalizing (depression, anxiety and posttraumatic stress) symptoms, as well as interpersonal risk and protective factors (affiliation with delinquent peers, parental involvement and teacher support). Hierarchical multiple binary logistic regression analysis was used to examine the associations between these variables and RSB. Results: Boys reported engaging in more RSB than girls. Externalizing symptoms and affiliation with delinquent peers were most strongly associated with RSB, whereas symptoms of anxiety were negatively associated with RSB. There was an interaction effect for sex and affiliation with delinquent peers on RSB with boys reporting RSB when having more delinquent peers. Neither parental involvement nor teacher support were protective against RSB. Conclusions: Early detection of and interventions for RSB and associated externalizing symptoms may be important for adolescent physical and mental wellbeing. Affiliation with delinquent peers should, especially among boys, be regarded as a risk marker for RSB.
Читать
тезис
|
Risky sexual behaviour among Russian adolescents: association with internalizing and externalizing symptoms
|
01.12.2021 |
Isaksson J.
Westermark C.
Koposov R.A.
Stickley A.
Ruchkin V.
|
Child and Adolescent Psychiatry and Mental Health |
10.1186/s13034-021-00393-3 |
0 |
Ссылка
Background: Risky sexual behaviour (RSB) is regarded as a major health problem during adolescence. Russia has one of the highest rates of teenage pregnancy, abortion and newly diagnosed HIV infections in the world, but research on RSB in Russian youth has been limited. To address this deficit, this study examined the role of several factors, including internalizing and externalizing symptoms, in RSB among Russian adolescents. Methods: Self-reported data were collected from 2573 Russian adolescents aged 13–17 years old (59.4 % girls; Mean age = 14.89) regarding RSB (unprotected sex, early pregnancy, multiple sexual partners and substance use during sexual encounters). Information was also obtained on externalizing (conduct problems and delinquent behaviour) and internalizing (depression, anxiety and posttraumatic stress) symptoms, as well as interpersonal risk and protective factors (affiliation with delinquent peers, parental involvement and teacher support). Hierarchical multiple binary logistic regression analysis was used to examine the associations between these variables and RSB. Results: Boys reported engaging in more RSB than girls. Externalizing symptoms and affiliation with delinquent peers were most strongly associated with RSB, whereas symptoms of anxiety were negatively associated with RSB. There was an interaction effect for sex and affiliation with delinquent peers on RSB with boys reporting RSB when having more delinquent peers. Neither parental involvement nor teacher support were protective against RSB. Conclusions: Early detection of and interventions for RSB and associated externalizing symptoms may be important for adolescent physical and mental wellbeing. Affiliation with delinquent peers should, especially among boys, be regarded as a risk marker for RSB.
Читать
тезис
|
On the application of different surfactant types to measure the carbonate’s adsorption density: a parametric study
|
01.12.2021 |
Peng X.
Aljeboree A.M.
Timoshin A.
Nassabeh S.M.M.
Davarpanah A.
|
Carbonates and Evaporites |
10.1007/s13146-021-00728-3 |
0 |
Ссылка
Due to the cost efficiency and environmentally friendly surfactant features in enhanced oil recovery techniques, the appropriate designation of surfactant flooding should be considered. It is essential to evaluate the crucial factors that affect surfactant adsorption on rock surfaces to eliminate the total economic losses of surfactant retention and adsorption in porous media. In this paper, the considerable influence of temperature, different surfactant concentrations, and polymer addition were experimentally investigated for dolomite minerals extracted from Pabdeh formation. According to this study, higher adsorption density has occurred at lower temperatures, which implies lower kinetic energy between the surfactant molecules. For 25 ℃, the adsorption density is about 41 mg/g, and it has the lowest value of 100 ℃. It is about 17 mg/g. By the increase of surfactant concentration for different time steps, adsorption density has been increased. For the surfactant concentration of 5 Wt. %, the adsorption density is about 42 mg/g; however, it is for 0.15 Wt. % of surfactant concentration, the adsorption density is about 1 mg/g. Moreover, due to the higher stability of polymers, adsorption density has been decreased by the addition of polymer. The stopping time for each surfactant concentration is about 6.5 h for the surfactant concentration of 5 Wt. %. Consequently, the critical micelle concentration point is about 3.5 Wt. %, 4 Wt. %, and 5 Wt. % for linear alkylbenzene sulfonic acid, cetyl trimethyl ammonium bromide, and Triton X-100, respectively. This change in the conductivity is related to the start of the micelling process by increasing surfactant concentration.
Читать
тезис
|