Репозиторий Университета

Год публикации: Все года 2018 2019 2020

Название	Дата публикации	Коллектив авторов	Журнал	DOI	Индекс цитирования	Ссылка на источник
A Rational Approach for Obtaining High-Specific Polyclonal Antibodies against Recombinant Alpha-Synuclein adjuvant alpha-synuclein immunization polyclonalantibodies	01.10.2018	Barinova K. Melnikova A. Schmalhausen E. Muronetz V.	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry		0	Ссылка © 2018, Pleiades Publishing, Ltd. Abstract: An approach for quick and efficient production of polyclonal antibodies to the target antigen, alpha-synuclein, has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund’s adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry. Читать тезис
A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein Adjuvant Alpha-synuclein Immunization Polyclonalantibodies	01.01.2018	Barinova K. Melnikova A. Schmalhausen E. Muronetz V.	Biomeditsinskaya Khimiya		0	Ссылка © 2018 Russian Academy of Medical Sciences. All rights reserved. The approach for the quick and efficient production of polyclonal antibodies to the target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry. Читать тезис

Репозиторий Университета

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