Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia
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01.03.2021 |
Dedkov V.G.
Dolgova A.S.
Safonova M.V.
Samoilov A.E.
Belova O.A.
Kholodilov I.S.
Matsvay A.D.
Speranskaya A.S.
Khafizov K.
Karganova G.G.
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Ticks and Tick-borne Diseases |
10.1016/j.ttbdis.2020.101612 |
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Ссылка
© 2020 Elsevier GmbH Wad Medani virus (WMV) belongs to the genus Orbivirus and is a poorly studied arbovirus with unclear medical significance. Presently, a limited number of WMV strains are characterized and available in NCBI GenBank, some isolated many years ago. A new WMV strain was isolated in 2012 from Dermacentor nuttalli ticks collected from sheep in the Tuva Republic, Russia, and sequenced using high-throughput methods. Complete coding sequences were obtained revealing signs of multiple intersegment reassortments. These point to a high variability potential in WMV that may lead to the formation of strains with novel properties. These new data on WMV can promote better understanding of: ecological features of its circulation; relationships within the genus Orbivirus; and the medical significance of the virus.
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Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia
|
01.03.2021 |
Dedkov V.G.
Dolgova A.S.
Safonova M.V.
Samoilov A.E.
Belova O.A.
Kholodilov I.S.
Matsvay A.D.
Speranskaya A.S.
Khafizov K.
Karganova G.G.
|
Ticks and Tick-borne Diseases |
10.1016/j.ttbdis.2020.101612 |
0 |
Ссылка
© 2020 Elsevier GmbH Wad Medani virus (WMV) belongs to the genus Orbivirus and is a poorly studied arbovirus with unclear medical significance. Presently, a limited number of WMV strains are characterized and available in NCBI GenBank, some isolated many years ago. A new WMV strain was isolated in 2012 from Dermacentor nuttalli ticks collected from sheep in the Tuva Republic, Russia, and sequenced using high-throughput methods. Complete coding sequences were obtained revealing signs of multiple intersegment reassortments. These point to a high variability potential in WMV that may lead to the formation of strains with novel properties. These new data on WMV can promote better understanding of: ecological features of its circulation; relationships within the genus Orbivirus; and the medical significance of the virus.
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Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution
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01.11.2019 |
Gaetani M.
Sabatier P.
Saei A.
Beusch C.
Yang Z.
Lundström S.
Zubarev R.
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Journal of Proteome Research |
10.1021/acs.jproteome.9b00500 |
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Ссылка
© 2019 American Chemical Society. Various agents, including drugs as well as nonmolecular stimuli, induce alterations in the physicochemical properties of proteins in cell lysates, living cells, and organisms. These alterations can be probed by applying a stability- and solubility-modifying factor, such as elevated temperature, to a varying degree. As a second dimension of variation, drug concentration or agent intensity/concentration can be used. Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. We envision widespread use of the PISA approach in chemical biology and drug development.
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