Репозиторий Университета

© 2018 International Society for Cellular Therapy Background: Menstrual blood is only recently and still poorly studied, but it is an abundant and noninvasive source of highly proliferative mesenchymal stromal cells (MSCs). However, no appropriate isolation method has been reported due to its high viscosity and high content of clots and desquamated epithelium. Methods: We studied three different isolation approaches and their combinations: ammonium-containing lysing buffer, distilled water and gradient-density centrifugation. We tested the proliferative capacity, morphology, surface markers and pluripotency of the resulting cells. Results: Our isolation method yields up to four million nucleated cells per milliliter of initial blood, of which about 0.2–0.3% are colony-forming cells expressing standard mesenchymal markers CD90, CD105 and CD73, but not expressing CD45, CD34, CD117, CD133 or HLA-G. The cells have high proliferative potential (doubling in 26 h) and the ability to differentiate into adipocytes and osteocytes. Early endometrial MSCs (eMSCs) express epithelial marker cytokeratin 7 (CK7). CK7 is easily induced in later passages in a prohepatic environment. We show for the first time that a satisfactory and stable yield of eMSCs is observed throughout the whole menstrual period (5 consecutive days) of a healthy woman. Discussion: The new cost/yield adequate method allows isolation from menstrual blood a relatively homogenous pool of highly proliferative MSCs, which seem to be the best candidates for internal organ therapy due to their proepithelial background (early expression of CK7 and its easy induction in later passages) and for mass cryobanking due to their high yield and availability.

© 2018 Izdatel'stvo Meditsina. All rights reserved. Anthropogenic physical, chemical, and biological impacts of objects on living organisms in the environment lead to profound structural and functional disorders primarily in the evolutionary current microbiocenosеs of a human. This is often accompanied by a deterioration in his health, an increase in the risk of infectious, chronic metabolic and mental diseases, which eventually can put a question for not only the preservation on our planet but also the entire existing diversity of living organisms. The development of cryobiotechnology and the creation of cryobanks of different directions is a common global trend in the development of Biological, Medical, Agricultural and Environmental Sciences. The advantage of the proposed technique of cryopreservation of symbiotic microbial associations is the storage of the biomaterials taken from the people will be carried out in an atmosphere of liquid nitrogen (-196°C) for the long-term storage. Fundamental and applied research on cryopreservation of microbiota of different human biotopes has a social orientation because they are directly related to human ecology and health of the nation.