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Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses
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01.01.2020 |
Dolgova A.
Safonova M.
Dedkov V.
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Methods in Molecular Biology |
10.1007/978-1-0716-0138-9_14 |
0 |
Ссылка
© Springer Science+Business Media, LLC, part of Springer Nature 2020. This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.
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