Репозиторий Университета

Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses


  • Dolgova A.
  • Safonova M.
  • Dedkov V.
Дата публикации:01.01.2020
Журнал: Methods in Molecular Biology
БД: SCOPUS
Ссылка: SCOPUS

Аннтотация

© Springer Science+Business Media, LLC, part of Springer Nature 2020. This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.


Вернуться назад