Репозиторий Университета

Год публикации: Все года 2018 2019 2020

Название	Дата публикации	Коллектив авторов	Журнал	DOI	Индекс цитирования	Ссылка на источник
Characterization and Detection of Erythropoietin Fc Fusion Proteins Using Liquid Chromatography-Mass Spectrometry bottom-up EPO-Fc fusionproteinidentification IdeS intactmassspectrometryanalysis middle-up signaturepeptide	05.01.2018	Mesonzhnik N. Postnikov P. Appolonova S. Krotov G.	Journal of Proteome Research		3	Ссылка © 2017 American Chemical Society. Erythropoietin Fc (EPO-Fc) fusion proteins are potential drug candidates that have been designed for the treatment of anemia in humans by stimulating erythrocyte production. Such compounds can be considered performance-enhancing agents that may be used by athletes in endurance sports. This study describes the primary structure of commercially available EPO-Fc based on comprehensive liquid chromatography coupled with mass spectrometry (LC-MS) analysis. A bottom-up approach and the intact molecular weight (MW) measurement of deglycosylated protein and its IdeS proteolytic fractions was used to determine the amino acid sequence of EPO-Fc. Using multiple proteases, peptides covering unknown fusion breakpoints (spacer peptides) were identified. We demonstrated that "spacer peptides" can be used in the determination of EPO-Fc fusion proteins in biological samples using common LC-tandem MS methods. Читать тезис