Extracellular matrix-based hydrogels obtained from human tissues: A work still in progress
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01.10.2019 |
Gazia C.
Tamburrini R.
Asthana A.
Chaimov D.
Muir S.
Marino D.
Delbono L.
Villani V.
Perin L.
Di Nardo P.
Robertson J.
Orlando G.
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Current Opinion in Organ Transplantation |
10.1097/MOT.0000000000000691 |
0 |
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© 2019 Wolters Kluwer Health, Inc. Purpose of reviewThe current review summarizes contemporary decellularization and hydrogel manufacturing strategies in the field of tissue engineering and regenerative medicine.Recent findingsDecellularized extracellular matrix (ECM) bioscaffolds are a valuable biomaterial that can be purposed into various forms of synthetic tissues such as hydrogels. ECM-based hydrogels can be of animal or human origin. The use of human tissues as a source for ECM hydrogels in the clinical setting is still in its infancy and current literature is scant and anecdotal, resulting in inconclusive results.SummaryThus far the methods used to obtain hydrogels from human tissues remains a work in progress. Gelation, the most complex technique in obtaining hydrogels, is challenging due to remarkable heterogeneity of the tissues secondary to interindividual variability. Age, sex, ethnicity, and preexisting conditions are factors that dramatically undermine the technical feasibility of the gelation process. This is contrasted with animals whose well defined anatomical and histological characteristics have been selectively bred for the goal of manufacturing hydrogels.
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Extrusion-based 3D printing of photocurable hydrogels in presence of flavin mononucleotide for tissue engineering
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01.01.2018 |
Savelyev A.
Sochilina A.
Akasov R.
Mironov A.
Semchishen V.
Generalova A.
Khaydukov E.
Popov V.
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Sovremennye Tehnologii v Medicine |
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4 |
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© 2018, Nizhny Novgorod State Medical Academy. All rights reserved. 3D printing became a widely used technique for tissue engineering applications. This additive technology enables easy fabrication of very complicated structures. However, selection and preparation of initial compositions for 3D printing satisfying high biocompatibility and processability requirements still remains challenging. One of the most promising materials for mimicking of the living tissues are hydrogels possessing properties close to native tissues. In this work, the printability of hydrogels based on hyaluronic acid and poly(ethylene glycol) derivatives dissolved in phosphate buffer saline in presence of flavin mononucleotide as an endogenous photosensitizer has been studied. To produce a hydrogel pattern, the extrusion of photocurable composition has been combined with its simultaneous photoinduced crosslinking under laser irradiation at 450 nm. Cytotoxicity of fabricated films and 3D scaffolds has been tested in vitro using human fibroblasts BJ-5ta.
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Hydrogel-assisted neuroregeneration approaches towards brain injury therapy: A state-of-the-art review
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01.01.2018 |
Kornev V.
Grebenik E.
Solovieva A.
Dmitriev R.
Timashev P.
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Computational and Structural Biotechnology Journal |
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3 |
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© 2018 The Authors Recent years have witnessed the development of an enormous variety of hydrogel-based systems for neuroregeneration. Formed from hydrophilic polymers and comprised of up to 90% of water, these three-dimensional networks are promising tools for brain tissue regeneration. They can assist structural and functional restoration of damaged tissues by providing mechanical support and navigating cell fate. Hydrogels also show the potential for brain injury therapy due to their broadly tunable physical, chemical, and biological properties. Hydrogel polymers, which have been extensively implemented in recent brain injury repair studies, include hyaluronic acid, collagen type I, alginate, chitosan, methylcellulose, Matrigel, fibrin, gellan gum, self-assembling peptides and proteins, poly(ethylene glycol), methacrylates, and methacrylamides. When viewed as tools for neuroregeneration, hydrogels can be divided into: (1) hydrogels suitable for brain injury therapy, (2) hydrogels that do not meet basic therapeutic requirements and (3) promising hydrogels which meet the criteria for further investigations. Our analysis shows that fibrin, collagen I and self-assembling peptide-based hydrogels display very attractive properties for neuroregeneration.
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