Tracing upconversion nanoparticle penetration in human skin
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01.12.2019 |
Khabir Z.
Guller A.
Rozova V.
Liang L.
Lai Y.
Goldys E.
Hu H.
Vickery K.
Zvyagin A.
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Colloids and Surfaces B: Biointerfaces |
10.1016/j.colsurfb.2019.110480 |
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© 2019 Elsevier B.V. Due to their unique optical properties upconversion nanoparticles (UCNPs) provide exceptionally high contrast for imaging of true nanoparticle distribution in excised human skin. It makes possible to show penetration of solid nanoparticles in skin treated with chemical enhancers. We demonstrated tracing upconversion nanoparticles in excised human skin by means of optical microscopy at the discrete particle level sensitivity to obtain their penetration profiles, which was validated by laser-ablation inductively-coupled-plasma mass-spectrometry. To demonstrate utilities of our method, UCNPs were coated with polymers, formulated in water and chemical enhancers, and applied on excised human skin mounted on Franz cells, followed by imaging using a custom-built laser-scanning microscope. To evaluate the toxicity impact on skin by polymer-coated UCNPs, we introduced a tissue engineering model of viable epidermis made of decellularized chick embryo skin seeded with keratinocytes. UCNPs formulated in water stopped in stratum corneum, whereas UCNPs formulated in ethanol-water solution crossed stratum corneum and reached viable epidermis – hence, the enhancement effect for solid nanoparticles was detected by optical microscopy. All polymer-coated UCNPs were found nontoxic within the accepted safety levels. The keratinocyte resilience to polyethyleneimine-coated UCNPs was surprising considering cytotoxicity of polyethyleneimine to two-dimensional cell cultures.
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Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models
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01.07.2018 |
Koloskova O.
Gileva A.
Drozdova M.
Grechihina M.
Suzina N.
Budanova U.
Sebyakin Y.
Kudlay D.
Shilovskiy I.
Sapozhnikov A.
Kovalenko E.
Markvicheva E.
Khaitov M.
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Colloids and Surfaces B: Biointerfaces |
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© 2018 Elsevier B.V. Development of efficient biodegradable, environmentally responsive, biocompatible and non-toxic delivery system is needed for efficient gene delivery. As well known, properties of the vehicle are determined by the structure of carrier components. The aim of the current study was to estimate in vitro transfection efficacy of aliphatic di-, tri- and tetrapeptide-based cationic lipoplexes loaded with siRNA in function of a number of cationic groups using 2D (monolayer culture) and 3D (multicellular tumor spheroids) in vitro models. Physicochemical properties and cytotoxicity of the liposomes were found to be dependent upon a number of amino acid derivatives in an amphiphilic polar head. Uptake of liposomes loaded with nucleic acid (lipoplexes) and their localization in HEK293T cells was studied by confocal microscopy. The liposomes based on lipotripeptides had the highest transfection efficiency which was 20-fold higher than those fabricated from lipotetrapeptides.
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Using a simple equation to predict the microporation-enhanced transdermal drug flux
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01.06.2018 |
Rzhevskiy A.
Telaprolu K.
Mohammed Y.
Grice J.
Roberts M.
Anissimov Y.
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European Journal of Pharmaceutics and Biopharmaceutics |
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© 2018 Elsevier B.V. The mathematical model describing drug flux through microporated skin was previously developed. Based on this model, two mathematical equations can be used to predict the microporatio-enhanced transdermal drug flux: the complex primal equation containing a variety of experimentally-determined variables, and the simplified straightforward equation. In this study, experimental transdermal fluxes of three corticosteroids through split-thickness human skin treated with a microneedle roller were measured, and the values of fluxes compared with those predicted using both the more complex and simplified equations. According to the results of the study, both equations demonstrated high accuracy in the prediction of the fluxes of corticosteroids. The simplified equation was validated and confirmed as robust using regression analysis of literature data. Further, its capability and ease of use was exemplified by predicting the flux of methotrexate through the skin microporated with laser and comparing with published experimental data.
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Difficulties in the diagnosis and treatment of tuberculosis pleural empyema complicated with chest wandering abscess, diaphragm destruction and penetration into the liver
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01.01.2018 |
Giller D.
Martel I.
Enilenis I.
Koroev V.
Kesaev O.
Giller B.
Bizhanov A.
Grigoryev Y.
Lavrov V.
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Russian Electronic Journal of Radiology |
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© 2018 Russian Electronic Journal of Radiology.All Rights Reserved. Purpose. To show the difficulty of diagnosis and treatment of atypical course of tuberculous empyema, when the process was complicated by tuberculosis of the chest, destruction of the diaphragm and penetration into the liver. Results. After chest MSCT scan, tuberculosis was suspected. The patient successfully underwent surgery: Pleurectomy with chest wandering abscess excision, resection of the VII rib and abdominal wandering abscess excision combined with the diaphragm and liver resection. The postoperative period was uncomplicated. During examination 4 years after surgery the patient was in a satisfactory condition, has ability to work, was transferred to group III dispensary tuberculosis registration. Discussion. Diagnosis and treatment of pleural empyema in some cases is difficult and, sometimes, requires differentiation from the oncological process. We have not found cases of tuberculous empyema spread of in the abdominal cavity with penetration into the liver in the literature. Conclusion. MSCT scan allowed to timely diagnose the atypical course of tuberculous empyema.
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Terahertz spectroscopy of immersion optical clearing agents: DMSO, PG, EG, PEG
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01.01.2018 |
Musina G.
Dolganova I.
Malakhov K.
Gavdush A.
Chernomyrdin N.
Tuchina D.
Komandin G.
Chuchupal S.
Cherkasova O.
Zaytsev K.
Tuchin V.
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Proceedings of SPIE - The International Society for Optical Engineering |
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3 |
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© 2018 SPIE. Application of terahertz (THz) spectroscopy for biological tissues is strongly limited by the extremely low penetration depth due to THz absorption by tissue water. One of the possible solution of such problem is the usage of THz wave penetration-enhancing agents (PEA) for optical clearing of tissues. In the present paper, the transmission-mode THz spectroscopy of a set of PEAs (polyethylene glycol with different molecular weight, propylene glycol, ethylene glycol, and dimethyl sulfoxide) was performed in order to reconstruct their dielectric properties and compare them with that of water. The obtained results emphasize the feasibility of using PEG to enhance the depth of THz wave penetration into tissues.
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