Репозиторий Университета

© 2018 Dynasty Publishing House. All rights reserved. The objective: To study M-CSF levels in venous blood and CSF1R gene polymorphism in women with tuboperitoneal infertility, who enter an IVF programme, and to assess their prognostic role. Patients and methods: The examination included 120 women, who were divided into two groups: 40 women with effective IVF (group I) and 80 women with ineffective IVF (group II). M-CSF serum levels were determined by ELISA method, the polymorphic marker rs386693509 of the c-fms gene UTR-3 region was examined by PCR method with subsequent Sanger sequencing. Results: Based on OR calculation, prognostically significant concentrations of M-CSF and c-fms genotype were found for diagnosing the development of pregnancy (95% CI = [1.934-11.116], p = 0.0021; 95% CI = [5.92-37.73], p = 0.0001, respectively). In women with effective IVF, weak correlation was found between M-CSF and the number of oocytes recovered by transvaginal puncture (TVP) (r = 0.3321, p = 0.0363). conclusion. The results of this work confirm a significant role of M-CSF in realisation of female reproductive function. The study has shown that serum M-CSF concentrations and polymorphism of its c-fms gene receptor have a predictive significance for pregnancy due to IVF procedure at the preconceptive stage. The obtained data contribute to our knowledge of the contribution of cytokines and gene polymorphisms to the development of an IVF pregnancy.