Репозиторий Университета

© 2018, Springer Japan KK, part of Springer Nature. Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C50-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95–97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58–64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.

© 2018, Dynasty Publishing House. All rights reserved. The objective. A comparative study of the dose uniformity, dose reproducibility, imprint areas on a planar simulation model as indicators of dispersion of spray-aerosol pharmaceutical forms of various trademarks for treatment of diseases of the mouth cavity and throat. Materials and methods. As objects of study we selected 10 drugs manufactured in the «aerosol» and «spray» forms. Test for dose uniformity was performed in accordance with articles of the State Pharmacopoeia of the Russian Federation «Aerosols and sprays», comparison of dose reproducibility was performed by comparing average dose mass dispersions according to Fisher. The test for the width of dispersion was performed by imprints obtained of a single release of drugs on filter paper with Dragendorff’s reagent and plates for thin-layer chromatography. Results. All metered-dose sprays successfully passed the test for dose mass uniformity. The test for dose mass reproducibility showed a high enough stability of dosage in all the examined drugs: the percentage error of the average dose mass was at the level 1–3%. The test for dispersion width performed on planar simulation models showed significant differences in the character of drug dispersion. For comparing the width of dispersion we calculated imprint areas from a single-dose release on PSM, which permitted to determine drugs with wide and fine dispersion as the most effective with respect to the way of drug delivery to the focus of inflammation – on the mucous membranes of the throat and mouth cavity. Conclusion. The optimal combination of the examined spray characteristics was found in the spray Tantum® Verde, which permits to recommend it as a drug of choice in paediatrics for local therapy of inflammatory diseases of the throat in children beginning from the age of 3 years.

© 2018, Dynasty Publishing House. All rights reserved. The objective. A comparative diagnosis of dispersion performance by the dynamic characteristics and dispersion composition of aerosol-spray forms of drugs for treatment of inflammatory disease of the throat in children (from the age of 3 years) and adults. Materials and methods. The objects of the study were 10 medications for treatment of inflammatory diseases of the throat, 9 of which have the drug form of a metered-dose spray and 1 drug has the form of undosed aerosol. A comparative diagnostics of the drugs was performed by the following dynamic spray characteristics: total length and length of particular spray phases, spray cone angle and a form of droplet population of the cone. The dispersion composition of spray was characterised by the mean and mass-median diameters of particles, particle size distribution. These characteristics were measured by a direct observation method – shadow photography – with digital processing of images using the «Actual Flow» software. Results. Diagnostics of spray dispersion by dynamic characteristics has shown that the most effective dispersion in the shape of a wide hollow cone was obtained in the drugs Tantum® Verde and Grammidin®. Diagnostics of sprays by their dispersion composition has confirmed the correspondence of all the examined preparations to pharmaceutical sprays for local treatment of the upper respiratory tract. Practically in all sprays, the maximal portion of the drug content is transferred by droplets of the optimal for spray sizes from 10 to 100 μm. The mass content of «hazardous» small droplets with sizes less than 10 μm is insignificant and amounts to less than 1%. Conclusion. Based on diagnostics of 10 sprays by the method of shadow photography on the basis of all examined parameters of the dynamics and fineness of dispersion the most effective dispersion from the viewpoint of drug delivery to throat mucous membranes is achieved in the drug Tantum® Verde.