Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
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15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
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International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
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тезис
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Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
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тезис
|
Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
Читать
тезис
|
Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
Читать
тезис
|
Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
Читать
тезис
|
Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
Читать
тезис
|
Effect of a radiolabel biochemical nature on tumor-targeting properties of EpCAM-binding engineered scaffold protein DARPin Ec1
|
15.02.2020 |
Deyev S.
Vorobyeva A.
Schulga A.
Abouzayed A.
Günther T.
Garousi J.
Konovalova E.
Ding H.
Gräslund T.
Orlova A.
Tolmachev V.
|
International Journal of Biological Macromolecules |
10.1016/j.ijbiomac.2019.12.147 |
0 |
Ссылка
© 2018 The Authors Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast for DARPins that internalize slowly after binding to cancer cells. The hypothesis was tested using DARPin Ec1 that binds to epithelial cell adhesion molecule (EpCAM). EpCAM is a promising therapeutic target. Ec1 was labeled with 125I using two methods to obtain the non-residualizing labels, while residualizing labels were obtained by labeling it with 99mTc. All labeled Ec1 variants preserved target specificity and picomolar binding affinity to EpCAM-expressing pancreatic adenocarcinoma BxPC-3 cells. In murine models, all the variants provided similar tumor uptake. However, 125I-PIB-H6-Ec1 had noticeably lower retention in normal tissues, which provided appreciably higher tumor-to-organ ratios. Furthermore, 125I-PIB-H6-Ec1 demonstrated the highest imaging contrast in preclinical models than any other EpCAM-imaging agent tested so far. In conclusion, DARPin Ec1 in combination with a non-residualizing label is a promising probe for imaging EpCAM expression a few hours after injection.
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Optical properties of porous polylactide scaffolds
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01.01.2018 |
Yusupov V.
Sviridov A.
Zhigarkov V.
Shubnyy A.
Vorobieva N.
Churbanov S.
Minaev N.
Timashev P.
Rochev Y.
Bagratashvili V.
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Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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0 |
Ссылка
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Light field intensity distribution in three-dimensional polylactide scaffolds after irradiation with low-intensity light from one side of the samples has been determined in the visible and near-infrared regions of the spectrum. Two different types of scaffolds manufactured by the methods of supercritical fluid foaming and surface selective laser sintering have been investigated. The problem is solved by numerical calculation according to the Monte Carlo method involving experimentally obtained information about effective optical parameters of the scaffold material. Information about intensity distribution of the incident light in the matrix volume is needed to assess the radiation level for the scaffold cells after photobiostimulation. It has been shown that the formation of the light field in case of strongly scattering media, such as polylactide scaffolds, is determined by anisotropy g and the scattering coefficient μs.
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Morphologic and chemical composition of particulate matter in motorcycle engine exhaust
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01.01.2018 |
Chernyshev V.
Zakharenko A.
Ugay S.
Hien T.
Hai L.
Kholodov A.
Burykina T.
Stratidakis A.
Mezhuev Y.
Tsatsakis A.
Golokhvast K.
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Toxicology Reports |
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10 |
Ссылка
© 2018 The Author(s) Despite the fact that environmental pollution due to motorcycle exhaust gases reports a great increase, motorcycle production exhibits a great increase through the last years. Countries of Asia and Africa are reported to be the major regions where two-wheeled vehicles are a major transportation mode, with tens of millions of units sold per year. Motorcycle exhaust particles are considered to be the major contributor to environmental pollution due to their airborne dispersion, containing great amount of polycyclic aromatic hydrocarbons (PAHs). This study aims at reporting an objective analysis of the main sources of the ambient air pollution as also particle size distribution and chemical composition analysis of particulate matter originated from the exhausts of two-wheeled vehicles used in the territory of Vladivostok, Russia. Various types of two-wheeled vehicles were examined (motorcycles, ATVs, scooters and wet bikes) using different types of engine and fuel system. Experimental results showed that there was no clear relation to the particle size distribution with the engine displacement of motorcycle and the number of strokes and the fuel system. Instead, there were reported two clear assumptions. The first one is that regarding to the motorcycle brand, a few samples did not exhibit a great percentage of PM10 fraction. The second one is that more modern vehicles, that have a harmful gas afterburning system, are usually the source of an increased percentage of PM10 emitted particles. At last, it should be mentioned that the laser particle size analysis method is capable of determining the particle sizes after their agglomeration whereas the optical morphometry method allows to determine the real particle size of emissions. In conclusion, it can be pointed out that the agglomeration of particles can lead to the reduction in the toxicity of particles emissions originated from two wheeled-vehicles.
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The genus Trollius (Ranunculaceae) in the Russian Far East
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01.01.2018 |
Luferov A.
Erst A.
Luferov D.
Shmakov A.
Wang W.
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Turczaninowia |
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1 |
Ссылка
© 2018 Altai State University. All rights reserved. The critical revision of Trollius L. (Ranunculaceae) in the Far East of Russia was made, in which nine species were recognized. The identifcation key and taxonomical synopsis of the genus have been provided. Synonymy, geographical distribution and coeno-ecological peculiarities of each species of these nine species are presented. For the frst time Trollius japonicus Miq. was found in the territory of Russia (the Kurile Islands: Iturup, Kunashir). Furthermore, we found that the information on the distribution of this species on Sakhalin Island is wrong owing to the incorrect identifcation. The information about the medical use of each of nine Trollius species is also provided.
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