Laser microsurgery of cell spheroids: An effective tool for regeneration studying and novel test system in aesthetic medicine
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13.08.2018 |
Kosheleva N.
Ilina I.
Zurina I.
Gorkun A.
Sitnikov D.
Saburina I.
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Proceedings - International Conference Laser Optics 2018, ICLO 2018 |
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0 |
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© 2018 IEEE. Technique of laser microsurgery of cell spheroids with nanosecond laser pulses was used to develop a new simple reproducible model for studying regeneration in vitro. Wound restoration accompanying the reparative processes occurred gradually over seven days due to rearrangement of surviving non-proliferating cells. Skin anti-ageing drugs can be tested on the developed model of cell spheroid's regeneration.
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2D/3D buccal epithelial cell self-assembling as a tool for cell phenotype maintenance and fabrication of multilayered epithelial linings in vitro
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18.07.2018 |
Zurina I.
Shpichka A.
Saburina I.
Kosheleva N.
Gorkun A.
Grebenik E.
Kuznetsova D.
Zhang D.
Rochev Y.
Butnaru D.
Zharikova T.
Istranova E.
Zhang Y.
Istranov L.
Timashev P.
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Biomedical Materials (Bristol) |
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3 |
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© 2018 IOP Publishing Ltd. Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling. Transitioning the cells from the monolayer to non-adhesive 3D culture conditions led to formation of self-assembling spheroids, with restoration of their epithelial characteristics after epithelial-mesenchymal transition. In 7 days, the cells within spheroids restored the apical-basal polarity, and the formation of both tight (ZO1) and adherent (E-cadherin) intercellular junctions was shown. Thus, culturing buccal epithelial cells in a 3D system allowed us to recover and durably maintain the morphological and functional characteristics of epithelial cells. The multilayered epithelial lining formation was achieved after placing spheroids for 7 days onto a hybrid matrix, which consisted of collagen layers and reinforcing poly (lactide-co-glycolide) fibers and was proven promising for replacement of the urothelium. Thus, we offer an effective technique of forming multilayered epithelial linings on carrier-matrices using cell spheroids that was not previously described elsewhere and can find a wide range of applications in tissue engineering, replacement surgery, and regenerative medicine.
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