Репозиторий Университета

© 2020 The Authors Regulated cell death (RCD) has a fundamental role in development, pathology, and tissue homeostasis. In order to understand the RCD mechanisms, it is essential to follow these processes in real time. Here, atomic force microscopy (AFM) is applied to morphologically and mechanically characterize four RCD modalities (intrinsic and extrinsic apoptosis, necroptosis, and ferroptosis) in murine tumor cell lines. The nano-topographical analysis revealed a distinct surface morphology in case of necroptosis, ∼ 200 nm membrane disruptions are observed. Using mechanical measurements, it is possible to detect the early onset of RCD. Combined elasticity and microrheology analysis allowed for a clear distinction between apoptotic and regulated necrotic cell death. Finally, immunofluorescence analysis of the cytoskeleton structure during the RCD processes confirm the measured mechanical changes. The results of this study not only demonstrate the possibility of early real-time cell death detection but also reveal important differences in the cytoskeletal dynamics between multiple RCD modalities.

© 2018 Izdatel'stvo Meditsina. All rights reserved. Anthropogenic physical, chemical, and biological impacts of objects on living organisms in the environment lead to profound structural and functional disorders primarily in the evolutionary current microbiocenosеs of a human. This is often accompanied by a deterioration in his health, an increase in the risk of infectious, chronic metabolic and mental diseases, which eventually can put a question for not only the preservation on our planet but also the entire existing diversity of living organisms. The development of cryobiotechnology and the creation of cryobanks of different directions is a common global trend in the development of Biological, Medical, Agricultural and Environmental Sciences. The advantage of the proposed technique of cryopreservation of symbiotic microbial associations is the storage of the biomaterials taken from the people will be carried out in an atmosphere of liquid nitrogen (-196°C) for the long-term storage. Fundamental and applied research on cryopreservation of microbiota of different human biotopes has a social orientation because they are directly related to human ecology and health of the nation.