Аннтотация

© 2018 Meditsina Publishers. All rights reserved. A method for producing recombinant peptidoglycan-associated lipoprotein (PAL) L. pneumophila in a heterologous expression system consisting of chimerical protein His-SUMO-PAL with hexa-histidine tag (for chromatographic purification of the precursor protein) and the protein PAL, which separated by SUMO protease peptide was developed. The method allows to obtain recombinant PAL with a high degree of purity and a yield of 0.3 g per liter of bacterial culture without fermentation. The obtained recombinant PAL retains the native structure, immunogenic properties and it can be used for vaccine development and production of monoclonal antibodies as a diagnostic tool at Legionella infection.